针刺调控铁代谢及p53/SCL7A11介导的氧化损伤抑制脑缺血再灌注大鼠铁死亡的作用和机制研究

铁死亡是脑缺血再灌注后神经损伤的重要机制，抑制铁死亡是治疗缺血性脑卒中的新思路。醒脑开窍针刺法临床治疗该病疗效显著，预实验发现针刺可调节铁代谢，提高脑组织抗氧化能力。因此提出醒脑开窍针刺法可通过调控铁代谢及p53/SCL7A11介导的氧化损伤抑制脑缺血再灌注大鼠铁死亡的假说。本研究拟以脑缺血再灌注大鼠为研究对象，将铁死亡理论用于针刺机理研究，采用普鲁士蓝染色、酶联免疫吸附测定法、逆转录PCR和实时荧光定量PCR技术进行检测。对比铁螯合剂（去铁胺），观察针刺后铁沉积量、铁代谢相关蛋白(铁蛋白、转铁蛋白、转铁蛋白受体1、膜铁转运蛋白1、活性氧自由基等)含量及相关mRNA的表达水平；对比p53激动剂（COTI2），观察针刺后p53/SCL7A11通路上p53、SLC7A11、谷胱甘肽过氧化物酶4、谷胱甘肽、丙二醛等含量变化以及相关mRNA的表达水平，探讨针刺作用并为针刺治疗提供理论指导和依据。

Ferroptosis is an important mechanism of nerve injury after cerebral ischemia reperfusion.Inhibiting ferroptosis is a new way to treat ischemic stroke.Previous studies have found that Xingnao Kaiqiao acupuncture therapy had a good effect. It was found that acupuncture therapy could regulate the expression of iron metabolism related proteins and enhance the antioxidant capacity in the pre-experiment.Therefore, it was hypothesized that Xingnao Kaiqiao acupuncture therapy could regulate iron metabolism and inhibit ferroptosis in rats with cerebral ischemia reperfusion by regulating of p53/SLC7A11 mediated oxidative damage.In this study,cerebral ischemia reperfusion rats were used as the reseach subjects,the theory of ferroptosis was applied in this study of acupuncture mechanism.Prussian blue staining,enzyme linked immunosorbent assay,reverse transcription-PCR and real-time quantitative polymerase chain reaction were used in this study.The iron deposition,the expression of iron metabolism related proteins (ferritin,transferrin,transferrin receptor 1,ferroportin 1,reactive oxygen species,etc.) and related mRNA were observed after acupuncture on cerebral ischemia reperfusion rats,comparing to iron chelator (desironamine) group.The expression of p53,SLC7A11,glutathione peroxidase 4,glutathione,malondialdehyde,etc in the p53/SCL7A11 pathway and the expression of related mRNA were observed after acupuncture,comparing to p53 agonists(COTI2) group.The aim of the study was to explore the effect of Xingnao Kaiqiao acupuncture therapy and provide theoretical guidance and basis for acupuncture treatment.