水稻MAPKKK蛋白DCR1对稻瘟病抗性的调控作用

MAPK途径参与植物的免疫反应，已报道OsMAPK3、OsMAPK6等MAPK蛋白参与稻瘟病抗性反应，但关于MAPKKK蛋白对稻瘟病抗性的调控作用却鲜有报道。利用EMS诱变水稻材料D802，我们获得了一份稻瘟病感病突变体dcr1，利用图位克隆的方法，我们发现突变体dcr1中编码MAPKKK蛋白的基因Dcr1因突变功能丧失后导致水稻感病。本项目拟在这些基础上，利用植物病理、植物生理、生物化学及分子生物学等相关技术手段，鉴定蛋白DCR1的激酶生化特性和功能，筛选DCR1的互作蛋白DAPs并对关键互作蛋白进行功能鉴定，分析DCR1的下游磷酸化底物及其对水稻稻瘟病抗性的调控作用。通过上述研究，揭示MAPKKK蛋白DCR1对稻瘟病抗性的调控作用，丰富植物免疫机理，为水稻等作物抗病育种提供理论依据和新策略。

MAPK pathway is involved in plant immune, and MAPK protein such like OsMAPK3 and OsMAPK6, have been reported to participate in rice blast resistance. However, the regulation of MAPKKK protein on rice immunity and its underlying mechanism remains largely unknown. Through EMS-mediated mutagenesis, we obtained a novel rice mutant dcr1, which is compromised in resistance against blast disease. By using the map-based cloning strategy, we successfully isolated Dcr1 gene which encodes a MAPKKK protein and found that a function-lose mutation of Dcr1 leads to compromised resistance to blast disease in the dcr1 rice. In this project, we plan to carry out the following studies: (1) To clarify the biochemical characteristics and functions of protein DCR1; (2) To Screen and identify DCR1 associated proteins (DAPs) and analysis of the functions of key DAPs; (3) To identify downstream substrate proteins phosphorylated by DCR1 and to determine their functions in regulation on immunity. Through these studies, we will uncover the molecular mechanism regulating blast resistance mediated by the MAPKKK protein DCR1. Because there are no similar reports in rice previously, our work will enrich the mechanism of plant immune and disease resistance, and provide theoretical basis and new strategies for rice resistant breeding.