石墨烯/MoS2异质结三维超材料的太赫兹传感器的构建及在大肠杆菌O157:H7检测中的应用研究

针对大肠杆菌O157:H7的感染不仅危害人类健康，还会导致严重社会经济损失，而现有检测方法存在诸多不足的问题，本项目提出基于石墨烯/MoS2异质结三维超材料的太赫兹传感器的大肠杆菌O157:H7检测方法。考察石墨烯/MoS2异质结的微观形貌、结构特征等参数信息及石墨烯/MoS2异质结超材料结构对太赫兹反射谱的影响，在理论上揭示石墨烯/MoS2异质结三维超材料的太赫兹辐射特性与物理机理，建立石墨烯/MoS2异质结的制备工艺、形貌特征及超材料结构参数与其太赫兹辐射特性关联机制；探明该太赫兹传感器与大肠杆菌O157:H7的特异性结合机理及基于纳米材料的太赫兹辐射-生物材料集成传感器的检测机理，建立基于石墨烯/MoS2异质结三维超材料结构和适配体的大肠杆菌O157:H7浓度的太赫兹信号感知模型，并揭示不同生存状态的大肠杆菌的浓度-介电常数-超材料的谐振峰频移及吸收强度的关联机制。本项目将突破快速、

Escherichia coli (E. coli) is usually infected by consuming unprocessed or contaminated food and can lead to the outbreak of large-scale infectious diseases, which is a great threat to human health, and make severe economic damnification to our society. In view of the shortcomings of the existing detection methods, the project proposed a novel terahertz aptasensor based on graphene/MoS2 heterojunction 3D metamaterial for the accurate, sensitive, rapid and simple detection of E. coli O157:H7. Firstly, controllable preparation of the graphene/MoS2 heterojunction 3D metamaterial will be studied, the mircro morphology, structural characteristics and terahertz performance of graphene/MoS2 heterojunction 3D metamaterial will be characterized. The effect of the graphene/MoS2 heterojunction and the geometry structure of the metamaterial on the terahertz reflectance spectrum were investigated. The terahertz radiation property and the physical mechanism of graphene/MoS2 heterojunction 3D metamaterials will be revealed. Further, the mechanism of this terahertz aptasensor in the target detection process, and the detection mechanism of terahertz radiation properties-biomaterial integration sensor based on nanomaterials will be studied. A terahertz aptasensing method of E. coli O157:H7 concentration signal based on graphene/MoS2 heterojunction 3D metamaterial will be established. Finally, the relationship among the different living status and dielectric constant of E. coli O157:H7, and the shift of the resonant peak of graphene/MoS2 heterojunction 3D metamaterial. This project will overcome the difficult problem of rapid, simple and high-sensitivity detection of E. coli O157:H7, and meanwhile, it will realize identification of live and dead E. coli O157:H7.