基于比较基因组学探究人体肠道可培养优势菌Faecalibacterium prausnitzii的遗传多样性

Faecalibacterium prausnitzii（简称Fp）是一种具有缓解炎症性肠病和代谢性疾病的微生物。研究表明，Fp广泛存在于健康人的肠道中，且在不同人群中具有不同的功能。但大规模的Fp基因组多样性研究鲜有报道。我们前期对中国人肠道中培养的Fp进行基因组研究发现Fp不同分离株基因组同源性较低。据此我们推测Fp具有高水平的基因组多样性。为了探究Fp基因组多样性，我们将对中国人肠道中培养获得的32株Fp分离株进行比较基因组和系统演化研究，采用基因组聚类、泛基因组、系统进化、KEGG功能、短链脂肪酸以及炎症细胞模型等生物信息学和体外功能实验多层次明确Fp分离株的遗传多样性，探讨不同个体来源的Fp菌株的系统演化规律，揭示Fp分类群在KEGG、短链脂肪酸合成以及细胞抗炎等生物学功能的差异。本项目将从纯培养的Fp基因组和功能研究作为出发点为Fp菌株精准应用于疾病的干预治疗提供理论依据。

Faecalibacterium prausnitzii（abbreviated to Fp）is a microorganism that can relieve inflammatory bowel disease and metabolic diseases. Studies have shown that Fp is widely colonizing in the human gut of healthy people and has different functions in different populations. However, there are few reports on large-scale genomic diversity of Fp. Our previous study on Fp isolated genomes from the gut of Chinese individuals found that different Fp isolates share a lower genome similarity. Based on this, we speculate that Fp has a high level of genomic diversity. In order to explore the genomic diversity of Fp, we will conduct comparative genome and phylogenetic studies on 32 Fp isolates cultured from the human gut of Chinese. we will use bioinformatics and in vitro functional experiments such as genome clustering, pan-genome, phylogenetic, KEGG annontatiom, short-chain fatty acids, and inflammatory cell models to determine the genetic and functional diversity of Fp isolates at multiple levels, to explore the systematic evolution of Fp strains from different individuals, reveal the differences in biological functions of Fp in KEGG, short-chain fatty acid synthesis, and cellular anti-inflammatory. This project will take the cultured Fp genome and function research as the starting point to provide theoretical basis for the precise application of Fp strains in disease intervention and treatment.