脑肠互动理论下振腹环揉法调控下丘脑CRH/CRHR1通路治疗慢性失眠的机制研究

课题组在前期脑肠互动理论研究下，证实腹部推拿提高了失眠患者下丘脑神经元活性，增强了下丘脑-海马区功能同步性，对5-HT、GABA等具有调节作用；改善了患者血清SP、NPY等脑肠肽紊乱，但尚缺少内在机制研究。下丘脑是人体生物钟调节中枢，其室旁核分泌的CRH可激活CRH/CRHR1信号通路，启动HPA轴，调节GABA、5-HT、SP等代谢产物，且这些产物又可通过脑肠肽上行通路影响CRH分泌，其效应满足脑肠互动理论，因此，课题组提出“振腹环揉法治疗失眠与调节脑肠肽，激活下丘脑CRH/CRHR1通路，启动HPA轴，进而调节COR、5-HT、GABA等代谢，维持神经内分泌稳态有关”的科学假说。通过完成实验动物行为学、分子生物学、形态学、代谢组学等检测，对比通路正常组和通路拮抗组指标异同，结合临床fMRI、MRS、1HNMR等检测，阐述腹部推拿治疗失眠与CRH/CRHR1信号通路的作用关系。

The research group confirmed that abdominal massage improved the activity of hypothalamic neurons, enhanced the synchrony of hypothalamic-hippocampal function, and had a regulatory effect on 5-ht, GABA, etc.The serum SP, NPY and other cerebral intestinal peptide disorders were improved.The hypothalamus is human body biological clock regulating center, the paraventricular nucleus CRH can activate the secretion of CRH/CRHR1 signal pathway, start the HPA axis, and then adjust the GABA, 5 - HT, SP and other metabolites, and can be accessed by the product and im brain neuropeptide uplink channel affect the secretion of CRH, its effect to satisfy brain gut interaction theory, therefore, the research puts forward "vibration knead abdominal ring method to treat insomnia and regulate ghrelin, activation of the hypothalamus CRH/CRHR1, start the HPA axis, and then adjust the COR, 5 - HT and GABA metabolism, maintain neuroendocrine steady-state about" scientific hypothesis.By completing the experimental animal behavior,molecular biology, and morphological tests, comparing the indicators of the normal pathway group and the pathway antagonism group, and combining with the clinical fMRI, MRS, 1HNMR and other tests, the functional relationship between abdominal massage treatment of insomnia and CRH/CRHR1 signaling pathway was expounded.