锑尾矿中硫氧化耦联锑还原过程的自养微生物驱动机制

由于锑矿区锑污染严重，对微生物的胁迫性强，加上营养物质匮乏，使得利用外源微生物或植物进行锑矿的生物修复很难开展。在锑矿尾矿中，土著微生物对原位环境有较高的适应性，且微生物可以通过呼吸作用将Sb(V)还原为Sb(III)，使锑更易与其他物质结合成更稳定的化合物，降低锑的迁移性，从而减少对周围环境的危害，因此，开展矿区土著微生物对极端污染环境的生态修复能力研究具有重要环境意义。针对尾矿金属浓度高、营养匮乏的特点，本项目聚焦尾矿中自养微生物驱动的硫氧化耦联锑还原这一全新的地球化学过程，拟利用稳定同位素示踪技术-宏基因组分箱技术研究锑尾矿中自养微生物驱动的硫氧化耦联锑还原过程机制，揭示该过程的生物地球化学相互关系，深入了解参与该过程的微生物种群、关键功能基因和代谢途径，以期描绘尾矿中自养型硫氧化耦联锑还原的微生物作用机制图，为开展矿区极端环境条件下金属污染的原位修复提供重要的理论依据。

The elevated metal toxicity and depleted nutrient content in mine tailings hinder the remediation practices using allochthonous microorganisms or plants. Comparing to allochthonous organisms, autochthonous organisms are adapted to the harsh tailing environments. In antimony mine tailings, indigenous microorganisms are capable of reducing Sb(V) to Sb(III) through respiration process, which decreases the mobility of Sb and thus restrains the Sb from contaminating larger areas. Therefore, application of indigenous microorganisms hold great potentials for remediation efforts in mine tailings. Since the mine tailings are typical oligotrophic environments with elevated metal contaminations, this proposal focuses on a novel aspect of the Sb biogeochemical cycle – Sb reduction fueled by S oxidizion process. The stable isotope probing will be used for the identification of the functional populations of the S-oxidizing Sb reducers. Subsequent metagenomic binning could decipher the functional traits and metabolic pathways of these functional populations. The current research is expected to provide essential knowledge and scientific guidances for future remediation practices of mine tailings.