基于T细胞受体CDR3编码基因测序探讨电针治疗慢性前列腺炎/慢性盆底疼痛综合症的免疫调节相关机制研究

慢性前列腺炎/慢性盆底疼痛综合症(CP/CPPS)是泌尿系最常见疾患，与自身免疫炎症相关，主要表现为长期反复的骨盆区疼痛及不同程度的尿频急尿痛等。研究表明T细胞参与了CP/CPPS的疾病进程，而TCR CDR3及CD4+CD25+Treg与T细胞功能直接相关。目前尚无免疫组库的方法探索其病理和治疗机制。本研究团队前期临床试验及循证学研究显示电针治疗CP/CPPS有效，优于假针。因此，电针可能通过调节TCR CDR3基因序列表达以及CD4+CD25+Treg比例，进一步调节T细胞的功能状态，从而发挥其临床抑制免疫性炎症的作用。本研究采用高通量测序技术，对CP/CPPS患者治疗前后外周血TCR CDR3测序，探索电针有效性与TCR CDR3多样性关系；同时，通过构建自身免疫性前列腺炎大鼠，观察电针前后大鼠外周血及前列腺组织CD4+CD25+Treg比例及FOXP3、相关细胞因子的表达水平。

Chronic prostatitis/chronic pelvic floor pain syndrome (CP/CPPS) is the most common disease of the urinary system, which is associated with autoimmune inflammation, mainly manifested by the long-term and repeated pelvic pain and varied degrees of urinary urgency, frequency and pain. Studies have shown that T cells are involved in the process of CP/CPPS, and TCR CDR3 and CD4+CD25+Treg are directly related to the function of T cells. However, there are no immune repertoire methods to explore the pathology and therapeutic mechanism of CP/CPPS. Our previous clinical trials and evidence-based studies have shown that electro-acupuncture was effective for CP/CPPS, and superior to sham electro-acupuncture. Therefore, electro-acupuncture might regulate the function of T cells through regulating the expression of TCR CDR3 gene sequence and the proportion of CD4+CD25+Treg; thus, to exert its clinical effect of inhibiting immune inflammation. In this study, the high throughput sequencing technology will be used to test the TCR CDR3 sequence in the peripheral blood of CP/CPPS patients before and after treatment, in order to explore the relationship between the effectiveness of electro-acupuncture and the diversity of TCR CDR3. Meanwhile, the proportion of CD4+CD25+Treg, the expression of FOXP3 and related cytokines in peripheral blood and prostate tissue of rats with autoimmune prostatitis will be observed before and after treatment.