中缝背核-基底外侧杏仁核环路参与痛相关负性情绪调节及电针干预

痛相关负性情绪是一种与组织损伤或潜在的组织损伤相关的不愉快的情感体验，疼痛患者中负性情绪的发生率高达70%。中缝背核（DRN）和基底外侧杏仁核（BLA）是调控疼痛和情绪的关键环节。电针可通过DRN缓解痛相关负性情绪，且已知DRN的5羟色胺（5-HT）能神经元向BLA发出投射，通过5-HT受体1A和2C调节BLA的兴奋/抑制平衡，因此电针可能通过DRN-BLA环路干预痛相关负性情绪的发生。本研究以弗氏完全佐剂足底注射建立慢性痛模型，观察痛反应和情绪行为，应用示踪、钙成像、光遗传和免疫荧光等技术观察DRN和BLA活性、5-HT受体表达和5-HT能神经元活性变化，明确慢性痛时DRN的5-HT能神经元引起1A和2C受体表达变化诱发BLA的兴奋/抑制失衡，形成负性情绪。在此基础上对模型动物进行电针干预，探讨电针通过DRN-BLA环路的哪些环节影响负性情绪的发生，揭示电针干预痛相关负性情绪的新机制。

Pain related negative emotion is an unpleasant experience caused by tissue injury or potential tissue injury, which displayed a high morbidity of 70% in patients suffering from pain. Dorsal raphe nuclei (DRN) and Basolateral Amygdala (BLA) are the keys of pain and emotion modulation. Electro-acupuncture (EA) can relieve pain related negative emotion via DRN, and the serotoninergic neuron projects into the BLA and regulates the exciting/inhibiting (E/I) balance via the 5-hydroxytryptamine (5-HT) receptor 1A and 2C. We hypothesize that DRN-BLA circuit is invovled in treating pain related negative emotion by the EA. We plan to establish a chronic pain model by plantar injection of Freund's Complete Adjuvant and observe the activity of DRN and BLA, the expression of 5-HT receptors and the activity of serotoninergic neuron, to explicit that serotoninergic neuron influence the E/I balance of BLA via 5-HT1AR and 5-HT2CR and induce negative emotion. Then the EA is applied on the animals to investigate how the EA prevents the happening of negative emotion through DRN-BLA circuit so as to reveal the new mechanism of the EA treating pain related negative emotion.