环境因子对挺水植物根系甲烷氧化与脱氮功能耦合的调控机制

根区脱氮甲烷氧化菌在湿地碳/氮循环中起着重要的桥梁作用。目前，挺水植物根系甲烷氧化与脱氮功能耦合机制及对环境因子的响应机理尚不清楚。因此，本项目拟以甲烷氧化活跃的湿地挺水植物根系为研究对象，以潜在的调控因素O2、CH4、NO3-、NO2-作为处理因子，设置“血清瓶-NMS培养无土植物根系-同位素标记”的室内控制实验，采用基于RNA的定量PCR、功能基因高通量测序，酶联荧光原位杂交等技术，识别和定位根系介导甲烷氧化与脱氮耦合的关键活性菌群。采用纯培养技术，检测纯菌株甲烷氧化与脱氮功能基因的表达特性，结合植物种类、生长阶段和植物根组织形态，揭示关键环境因素对挺水植物根系甲烷氧化与脱氮功能耦合的调控机制，研究结果可望在理论上有所突破，可为减缓湿地温室气体释放和水体富营养化治理技术研发提供科学依据。

Denitrifying methantrophs of plant root form an important bridge between the global carbon and nitrogen cycles in wetland. The root zone (root and rhizosphere) of wetland plants is under aerobic conditions and allows the growth of aerobic methanotrophs. However, it remains unclear that coupling mechanism of methane oxidation and denitrification by denitrifying methantrophs and their response to environmental factors in emergent plant roots. We wanted to address active and mechanism of methane oxidation and denitrification by aerobic methanotrophs and how specific environmental conditions select for this coupling process in emergent plant roots. The present study examine (i) detect the active denitrifying methantrophs and determine the related roles to methane oxidation and denitrification; (ii) explore the underlying coupling mechanism of methane oxidation and denitrification and their environmental controls using isotopic tracing technique, pure culture technique, functional gene abundance, high throughput sequencing, RNA-seq, and and network analyses. Moreover, the research will provide theoretical basis for apply plant microbes symbioses to resolve the problems of CH4 emission and wetlands eutrophication.