海洋酸化影响菲律宾蛤仔胚壳发生的机理研究

海洋酸化问题在我国近岸海域日益加剧，对海洋贝类的生存及养殖构成了极大威胁。胚壳是贝类幼虫重要的钙化保护组织，其发生受海洋酸化影响最为显著。我们前期研究发现，海洋酸化对胚壳发生造成的损伤甚至会跨世代影响到子代，并对种群繁衍影响显著。然而，海洋酸化影响胚壳发生的机理尚未阐明，使得评估和预测海洋酸化对贝类的影响及其生态和资源效应面临巨大的不确定性。本项目以菲律宾蛤仔为研究对象，运用多学科研究方法（荧光成像动态监测技术、稳定同位素示踪和反演技术、蛋白组学分析、CLARITY透明化技术、免疫组化技术等）,探明海洋酸化条件下胚壳发生的动态响应过程；揭示海洋酸化影响胚壳碳酸钙沉积的机制；阐明胚壳有机基质形成对海洋酸化的时空响应特征；通过集成分析，解析海洋酸化对胚壳发生的影响机理。预期结果将为精准评估海洋酸化对贝类早期发育阶段的影响及种群数量变动和群落结构变化的预测提供科学依据，并对贝类养殖具有指导意义。

Ocean acidification (OA), driven by increasing atmospheric carbon dioxide absorption and eutrophication, is developing rapidly in Chinese coastal waters. OA can severely affect marine bivalves, especially their larval stages. The formation of initial larval shell (termed prodissoconch) is a significant hurdle for survival and further development during rapidly calcifying larval stages, therefore particularly sensitive to ongoing OA. Our previous results showed that the negative effects of OA on larval prodissoconch can generate carry-over effects on subsequent life-history stages and also persist into the next generation, severely affecting the recruitment and population maintenance. However, underlying mechanisms by which OA affects the formation of prodissoconch remain poorly understood. The proposed study will take the Manila clam (Ruditapes philippinarum), an ecologically and economically significant bivalve species worldwide as a model, and adopt an interdisciplinary approach to close this gap in knowledge. More specifically, a cutting-edge fluorescence-based imaging and monitoring method will be applied to depict the simultaneous deposition of CaCO3 and organic matrix in situ under acidified conditions, and then several state-of-the-art methods will be used to decipher the underlying mechanisms. By integrated analysis methods, the project will gain a better understanding of how OA affects the formation of prodissoconch. Outcomes of the project will open a new avenue in accurately assessing and predicting the OA effects on marine bivalves and their implications for marine ecosystems and resources, and provide a practical guidance for future shellfish aquaculture.