多组学联合解析BbRho4调控球孢白僵菌生长及抗氧化力的分子机制

球孢白僵菌是研究和应用最广泛的生防真菌之一，能有力保障粮食作物高产稳产和安全生产。在应用过程中，生长和抗逆是衡量球孢白僵菌生防潜能的重要指标，而真菌Rho蛋白被证明对细胞生长和抗逆具有调控作用。申请者结合前期基因缺失菌株表型试验和胞内基因转录水平分析，提出“BbRho4可能通过调控多条代谢通路影响球孢白僵菌生长和抗氧化力”的科学假设，并拟在此基础上，进一步检测菌株代谢组和蛋白质组，通过与转录组联合分析，筛选可能与BbRho4协同调节菌株生长及抗氧化力的关键代谢物、蛋白或基因，进而利用添加目标代谢物增强剂/抑制剂和构建基因突变库表型试验，验证关键代谢物或蛋白生物学功能。此外，通过酵母双杂交确认BbRho4直接作用底物，最终揭示BbRho4调控球孢白僵菌生长及抗氧化力的分子机制。项目实施有望为代谢或基因工程手段改造菌株提供靶标代谢物或基因，同时为优化微生物杀虫剂并提升其应用潜力提供新思路。

Beauveria bassiana is one of the most widely studied biocontrol fungi, which applied to improve the production and safety of crops. Growth and anti-stresses ability are two important parameters to measure biocontrol potential of the biocontrol microbes during application. Rho proteins in fungi are proven to be involved in regulating cell growth and anti-stresses ability. We propose that“BbRho4 may affect the growth and antioxidant capacity of Beauveria bassiana by regulating multiple metabolic pathways”based on our preliminary phenotype test and transcriptome data of ΔBbrho4. Base on the previous work, the propose project intends to select the differentially expressed metabolites, proteins, or genes in ΔBbrho4 compared to those in the wild type strain by detecting the metabolomes and proteomes followed with multi-omics analyzing with the transcriptome data. Experimental facilitation or suppression of target metabolite production, and construction of gene mutation gene mutation library will be carried out to verify biological functions of key metabolites or protein. Additionally, yeast two-hybrid assay will be applied to determine the interaction between BbRho4 and the substrates protein in vivo. Our work aimed to reveal the mechanism of BbRho4 regulating the growth and antioxidant capacity of Beauveria bassiana. The knowledge gained from our work is expected to provide target metabolites or genes for biological modification of Beauveria bassiana by metabolic or genetic engineering methods, and propose new ideas for optimizing microbial insecticides and enhancing their application potential.