可注射型类人胶原蛋白骨缺损修复支架材料的构建与关键技术研究

本研究旨在构筑一种新型的具有良好生物相容性和骨缺损修复性能的可注射型类人胶原蛋白骨缺损修复支架材料。实验利用己二酸二酰肼对类人胶原蛋白（HLC）分子接枝改性，利用高碘酸钠对多糖（PS）分子进行醛基化改性，然后基于Schiff base反应使改性蛋白与多糖发生分子间交联构筑可降解的注射型骨缺损修复支架。为了实现支架材料与缺损组织间力学匹配、支架材料的降解速率可控和进一步强化支架的促新骨生成性能，在支架材料中加入了多元离子杂化的超长超细（亚）纳米线性羟基磷灰石和骨生长因子。.本研究将对可注射型支架材料的可注射性、固化速率，塑型后复合支架的微观结构、热力学性质，细胞毒性，降解速率，成骨性能等进行深入研究，并采用外周骨定量 CT（pQCT）扫描动态监控骨缺损修复状况，获取四维骨骼缺损修复发展变化展示，实现评价和反馈优化可注射型骨缺损修复支架材料对骨缺损组织修复的性能。

The purpose of this study is to construct a new injectable human like collagen scaffold with good biocompatibility and bone defect repair performance. By grafting with Diacylhydrazine adipate, human like collagen (HLC) molecules were endowed with hydrazide bond, and Polysaccharide (PS) molecules were oxidized by sodium periodate to have aldehyde group. Then, based on Schiff base reaction, a biodegradable injectable bone defect repair scaffold was constructed by crosslinking the modified protein and polysaccharide molecules. In order to match the mechanical properties of scaffolds with the defect tissue, control the degradation rate of scaffolds and further enhance the osteogenic properties of scaffolds, the ultra-long ultra-fine (sub)nano linear hydroxyapatite and bone growth factor were added into the system..In this study, the injectability and solidification rate of the injectable scaffold materials, the microstructure, thermodynamic properties, cytotoxicity, degradation rate and osteogenesis of the composite scaffold after solidification will be studied in depth. In order to evaluate and optimize the injectable scaffolds for bone defect repair, the dynamic monitoring of bone defect repair was carried out by using the peripheral bone quantitative CT scan (pQCT).