BvFVE1通过调节组蛋白去乙酰化修饰调控甜菜春化作用的机制研究

甜菜对春化作用的敏感程度是决定生长期长短的重要因素之一。现阶段研究表明组蛋白修饰是植物剂量春化作用时间的重要方式。然而,甜菜中组蛋白修饰如何参与调控春化作用目前尚未阐明。前期研究发现春化过程中BvFVE1表达模式与抽薹农艺性状变化显著相关。并且，BvFVE1与开花相关基因(BvELF3、BvBTC1)和组蛋白去乙酰酶(BvHDT1)具有共表达趋势。这些结果暗示BvFVE1介导的组蛋白去乙酰化修饰参与甜菜抽薹调控与春化时间剂量。为了验证这一假设，本项目拟从以下方面开展研究：（1）鉴定与BvFVE1相互作用的组蛋白去乙酰化酶（2）鉴定BvFVE1介导的春化相关组蛋白去乙酰化修饰位点（3）鉴定这些乙酰化修饰位点对春化基因表达的影响。本研究旨在揭示表观遗传调控甜菜春化作用的分子机制，为选育早播甜菜品种提供新思路。

Vernalization sensitivity is one of the important factors that determine the growth period in sugar beet. Recent studies suggest that counting the dosage of vernalization are highly depend on epigenetic regulation. However, how histone acetylation regulating vernalization in sugar beet has not been clarified. Our previous study presented that the expression pattern of BvFVE1 was significantly correlation with the change of bolting agronomic traits during vernalization. Additionally, BvFVE1 was co-expressed with flowering-related genes (BvELF3 and BvBTC1) and histone deacetylase (BvHDT1). It implied that BvFVE1-mediated histone deacetylation plays role in bolting regulation and the dosage of vernalization time in sugar beet. To test this hypothesis, following aspects will be studied: (1) Identifying the interaction between BvFVE1 and HDACs; (2) Identifying the vernalization-related histone deacetylation sites mediated by BvFVE1; (3) Identifying vernalization-related genes regulated by histone deacetylation. The purpose of the study was to reveal the epigenetic mechanisms of vernalization in sugar beet and to provide new ideas for breeding of early sown sugar beet varieties.