动态程控仿真生长猪胃小肠消化吸收快速定量饲料氨基酸消化率的研究

我国生长猪年消耗的蛋白质高达3000万吨，精准、快速地评定生长猪对饲料蛋白质（氨基酸）的消化率是实现蛋白质资源高效利用的关键。本研究采用酶学、质谱和统计学技术和方法，重点研究：（1）饲粮组成对生长猪胃和小肠各位点消化液组成、食糜特性和停留时间的影响，解析猪体内蛋白质消化环境的变异规律，为模拟蛋白质消化提供生物学靶标；（2）动态仿真蛋白质在生长猪胃肠道消化的方法研究，阐明与体内消化条件匹配的动态仿真消化的参数及模拟消化方案，达到缩小模拟消化与体内消化程度差异的目标；（3）模拟消化法估测生长猪对饲料氨基酸标准回肠消化率的有效性研究，进一步从方法学的角度验证所建立的模拟消化法是否可以用于猪饲料氨基酸消化率的快速估测。预期结果将阐明猪胃、小肠蛋白质的代谢规律，建立动态仿真生长猪胃小肠消化吸收快速定量氨基酸消化率的方法，为国家实施的养殖业“蛋白质减量”、“豆粕替代”计划提供技术支撑。

The annual consumption of feed protein by growing pigs is as high as 30 million tons in China. Accurate and rapid assessment of the digestibility of feed protein or amino acids of growing pigs is critical for improving the utilization efficiency of protein resources. Using the methods of enzymology, mass spectrometry, and statistical techniques, this project focuses on the following research: (1) The effect of diet composition on the characteristics of digestive fluid and chyme, and retention time in the stomach and small intestine of growing pigs. Analyze the variation of the protein digestion environment in pigs, and provide biological targets for simulating protein digestion. (2) Study on the method of dynamically simulated protein digestion in the gastrointestinal tract of growing pigs, and clarify the parameters and simulated digestion scheme to dynamic simulate gastrointestinal digestion that matches the digestion conditions in the pig, and achieve the goal of reducing the difference between simulated and in vivo digestion. (3) The effectiveness of the simulated digestion method to estimate the standardized ileal digestibility of feed amino acids for growing pigs, which was further verified from a methodological point whether the established simulated digestion method can be used for the rapid and accurate estimation of the digestibility of feed amino acid for pigs. The expected results will clarify the protein metabolism in pig stomach and small intestine, establish a dynamic computer-controlled bionic system to quickly determine the digestibility of amino acids by simulating the digestion and absorption of the stomach and small intestine of growing pigs and provide technical support for the national implementation of the “protein reduction program” and “soybean meal replacement program” in the livestock industry.