SlMAPK3调控RNAi通路关键蛋白DRB抗番茄黄化曲叶病毒（TYLCV）分子机制研究

番茄黄化曲叶病毒（TYLCV）严重危害我国乃至世界番茄安全生产。RNAi在植物抗病毒中发挥重要作用，双链RNA绑定蛋白（DRB）对RNAi的启动至关重要。我们研究发现过表达MAPK级联关键基因SlMAPK3增强RNAi通路关键蛋白DRB的表达，提高番茄植株抗性，目前关于SlMAPK3调控RNAi通路关键蛋白DRB抗病毒机制仍不清楚。本项目在完成SlMAPK3调控番茄抗TYLCV研究基础上，拟研究SlMAPK3调控RNAi关键蛋白DRB抗TYLCV分子机制，（1）筛选被SlMAPK3调控的DRB，（2）确定与SlMAPK3互作的DRB蛋白，（3）验证DRB在SlMAPK3抗TYLCV中的功能，明确番茄MAPK级联信号与RNAi通路关系，揭示SlMAPK3与RNAi通路关键蛋白DRB互作机制，阐明SlMAPK3调控RNAi关键蛋白DRB抗病毒的分子机制，为番茄抗病毒研究奠定理论基础与实践意义。

Tomato yellow leaf curl virus (TYLCV) seriously threatens the safe production of tomato in China and the world. RNA interference（RNAi）plays an important role in plant antiviral defense, and double-stranded RNA binding protein (DRB) is essential for the priming of RNAi. It was found that overexpression of MAPK cascade key kinase SlMAPK3 enhances the expression of DRB, a key protein in the RNAi pathway, and improves the resistance of tomato plants. However, It remains unclear about the antiviral mechanism of SlMAPK3 in regulating the key protein of DRB in RNAi pathway protein. Based on the study of SlMAPK3 regulating tomato anti-TYLCV, this project plans to study SlMAPK3 regulating RNAi-key protein DRB antiviral, (i) screening DRB regulated by SlMAPK3, (ii) determining the interaction between DRB and SlMAPK3, (iii) verification the function of DRB in SlMAPK3 anti-TYLCV. This study will clarify the relationship between tomato MAPK cascade signal and RNAi pathway, reveal the interaction mechanism between SlMAPK3 and the key protein DRB in RNAi pathway, clarify the molecular mechanism of SlMAPK3 regulating RNAi key protein DRB against TYLCV, which will provide an important theoretical and practical basis and for tomato antiviral research.