拟南芥BRCT结构域蛋白SDSB1调控DNA双链断裂修复的机理

所有生物都进化了复杂而精细的DNA损伤修复系统以维持基因组的稳定性。在不同类型的DNA损伤中，DNA双链断裂（DNA double strand breaks, DSB）对细胞危害最严重。植物调控DSB修复的机理还有待于进一步研究。在前期的研究中，我们筛选到一个对DSB诱导试剂非常敏感的拟南芥突变体sdsb1。我们克隆了SDSB1基因，发现它编码一个含有BRCT（BRCA1 C-terminal）结构域的蛋白，但其生物学功能尚无报道。在此基础上，本项目将通过鉴定SDSB1的相互作用蛋白和筛选sdsb1的抑制子等策略研究SDSB1调控DSB修复的机理。本项目将发现植物调控DSB修复的新组分（SDSB1、其互作蛋白及抑制子基因）和新机制，具有重要的科学意义。

All organisms have evolved with complicated and sophisticated DNA damage repair system to maintain genome stability. Among various types of DNA damage, DNA double strand breaks (DSB) is the most serious for cells. The mechanisms underlying DSB repair in plants require more studies. In our previous studies, we identified an Arabidopsis mutant sdsb1, which is hypersensitive to DSB-inducing agents. We cloned SDSB1 and found that it encodes a protein containing BRCT (BRCA1 C-terminal) domain. The biological function of SDSB1 has not been reported yet. Based on these results, we will study how SDSB1 regulates DSB repair by identification of SDSB1-interacting proteins and screening the suppressors of sdsb1 mutant. This study will help to discover new components (SDSB1, its interacting protein, and the suppressor genes) and new mechanisms involved in DSB repair in plants, which is of important scientific significance.