拟南芥中水杨酸介导花粉管生长的受体筛选及其分子机理研究

水杨酸(SA)是一类非常重要的植物激素，不仅参与了植物的免疫应答反应，同时也调控了植物的生长发育。目前对SA介导植物免疫应答的机理已有一定的了解，但对SA调控植物生长的分子机理尚不清楚。花粉管是一个极性的单细胞，其生长受到细胞内外信号的调控，其中ROP1是花粉管极性生长的中心调控因子。最近研究结果发现SA通过调控花粉管的内吞和ROP1的活性从而抑制拟南芥花粉管的生长，但不依赖于已知的SA 受体NPR1/3/4，这说明植物体内存在其他SA受体调控花粉管的生长，那么SA是如何调控花粉管生长的呢？本项目拟将通过生化和反向遗传学的方法筛选SA调控拟南芥花粉管生长的受体，并利用分子生物学的技术手段研究SA受体的功能揭示SA调控植物生长的分子机理，为培育即抗病又高产的作物新品种提供理论。

It has reported that SA (Salicylic acid) is a vital phytohormone not only regulating plant immunity but also growth and development. At present, the mechanism of plant immune response mediated by SA has been understood to some extent. However, the mechanism of SA regulating plant growth is far from clear. Pollen tube, a single cell, is regulated by both intracellular and extracellular signal. ROP1 is known as a key player regulating pollen tube tip growth. Recent results shows that SA can regulate pollen tube growth through ROP1 and endocytosis independent of the traditional receptors NPR1/NPR3/NPR4, which indicates that there may exist other unknown SA receptors in plants. How does SA regulate pollen tip growth? Therefore, this proposal is going to screen the SA receptors regulating pollen tube tip growth through reverse genetic method and biochemistry techniques and to study the function of SA receptors to explain the molecular mechanism of SA regulation plants growth， which may provide a theory for breeding new crop varieties with both disease resistance and high yield.