利用基因编辑梅山猪新种群揭示Wip1基因调控猪脂肪生成的机理

猪脂肪性状与肉品质和饲料转化率密切相关，脂肪生成机制研究具有重要科学意义。野生型p53诱导磷酸酶1（Wip1）是重要调控因子，其对免疫和繁殖的调节作用研究较多，但在调控小鼠脂肪生成中的重要功能刚刚受到关注，且调控猪脂肪性状的功能未见报道。在前一项国家基金资助下，本团队获得了Wip1基因编辑猪，揭示了调控猪繁殖性状的机制，并发现其脂肪沉积明显减少。本项目将利用本团队独有的Wip1基因编辑梅山猪新种群，针对Wip1磷酸酶，采用dDIA磷酸化蛋白质组学新技术，规模化获取脂肪组织差异磷酸化位点及其对应蛋白信息，通过其与Wip1的互作及与猪脂肪表型性状的关联分析，阐明Wip1调控目标蛋白特异位点磷酸化从而影响脂肪生成的机制。本项目以在脂肪沉积方面具有代表性的我国优良地方种质资源为素材，在国内外同行大量前期工作基础上，阐明Wip1调控猪脂肪生成的新机制，为培育优质高效猪新品种提供新的分子基础。

The fat traits of pigs are closely related to meat quality and feed conversion, and the study of fat formation mechanism is of great scientific significance. Wild-type p53-induced phosphatase1 (Wip1) is an important regulatory factor, which has been widely studied in the regulation of immunity and reproduction. Its important function in the regulation of mouse adipogenesis has just been concerned, but its function in the regulation of pig adipogenesis has not been reported. With the support of the former grant of National Natural Science Foundation of China, our team obtained the editing pig of Wip1 gene, revealed the mechanism of regulating the reproductive traits of pigs, and found that the fat deposition of editing pigs was significantly reduced. In this project, by using our team's unique new genetic resources of Wip1 gene editing Meishan pig, and aiming at the fact that Wip1 is phosphatase, we will use direct data independent acquisition（dDIA） phosphorylation proteomics new technology to obtain the information of different phosphorylation sites and their corresponding proteins in fat tissues on a large scale. Through the analysis of interaction with Wip1 and the correlation analysis with the fat phenotype of pigs, we will clarify that the specific phosphorylation of the target proteins regulated by Wip1 and then their regulation mechanism of fat formation. In this project, based on the representative local germplasm resources of our country in terms of fat deposition and a large number of high-level previous work of our colleagues at home and abroad, the new mechanism of regulating fat production of pigs by Wip1 will be clarified, which will provide a new molecular basis for the cultivation of high-quality and efficient new varieties of pigs.