基于TBTV P3和TVDV运动相关蛋白互作的复合侵染协同移动机理研究

TBTV与TVDV作为烟草丛顶病的主要病原物，两者具有特殊的关系，TBTV依赖TVDV外壳蛋白的异源包裹才能被蚜虫传播，然而这两种病毒在植物体内的移动和运输机理并不清楚。我们前期研究发现接种1天后在非接种叶TBTV和TVDV复合侵染组合中各自的病毒积累量均高于两者单独侵染，同时TBTV P3与TVDV多个移动相关蛋白互作，TBTV改变了TVDV CP的亚细胞定位模式。以上结果暗示，在TBTV和TVDV复合侵染过程中，两者可能通过蛋白之间的互作在病毒的移动和运输中发挥了一定的调控作用。本项目拟通过免疫组织定位、免疫电镜、双分子荧光互补、免疫共沉淀及质谱分析等技术分析TBTV和TVDV病毒复合体的组织定位场所及运动方式，移动蛋白的互作方式以及移动复合体的组分等问题，解析TBTV和TVDV复合侵染协同移动机制。研究结果有助于更深入了解复合侵染中病毒-病毒-寄主之间的互作机理以及协同进化关系。

Tobacco bushy top virus (TBTV) and Tobacco vein distorting virus (TVDV) are the two major causal agents of tobacco bushy top disease. There are special relationships between TBTV and TVDV, TBTV relies on the coat protein of TVDV for aphid transmission. However, The collaborative movement mechanism during co-infection of TBTV and TVDV is not clear. Our previous studies confirmed that synergistic infection of TBTV and TVDV increased the accumulation of both their RNAs in non-inoculated leaves by 1 days post inoculation. Moreover, We found that TBTV P3 protein interacts with multiple movement related proteins of TVDV in vivo, the co-infection of TBTV altered the TVDV CP subcellular localization model. These results suggested that in the process of TBTV and TVDV synergistic infection, the direct interaction between TBTV encoded protein and TVDV encoded protein played a special role in the transportation of the two viruses. Based on these results, this project to analysis the location and movement ways of TBTV and TVDV complex movement components, the function of movement protein interactions and the components of complex by variety of molecular biology technology such as immune histochemical localization, immune electron microscopy (ISEM), BIFC, immune co-precipitation, and mass spectrometry. Then, to explore the movement mechanism between TBTV and TVDV co-infection. Research results will help us further to understand the interaction mechanism among virus-virus-host, and co-evolution model during the coevolution of virus and virus.