基于3-羟基黄酮的无金属光控型CO释放剂分子的研究

CO是人类发现的第二种内源性气体信号分子，在许多重要的生理和病理过程中发挥着重要作用，并呈现出广阔的临床应用前景。然而，由于缺乏安全有效的体内CO输送和给药方式，如何实现CO气体临床治疗成为极富挑战性的难题。发展无金属的CO释放剂分子有望解决这一问题，因此开展这方面的研究具有重要的意义。本项目拟在申请人近年来发展的一系列CO荧光探针的基础上，以3-羟基黄酮为母体分子，通过引入另一荧光团及其它定位基团，生物活性物种介导基团等，来构建新颖的无金属光控型CO释放剂分子，并研究它们在生命体系中释放CO的性能，以及应用活体模型来研究它们在疾病治疗中的应用。本项目具有良好的研究基础和工作条件，并已论证了可行性。项目的实施有望发展出多种集可控释放、荧光成像、定位和治疗一体化的无金属CO释放剂分子，从而为生物体内安全有效输送CO和CO气体治疗提供新的解决途径。

Carbon monoxide (CO) is the second endogenous gasotransmitter discovered by humans, which plays an important role in many important physiological and pathological processes, and presents a broad clinical application prospect. However, due to the lack of safe and effective CO delivery and administration methods in vivo, it is very challenging to achieve CO-based clinical treatment. The development of metal-free CO-releasing molecules is expected to provide a solution to this issue, and hence of great research significance. Based on the applicant's recent development of a series of CO fluorescent probes, this project aims to construct novel metal-free light-controlled CO-releasing molecules, study their performance in the release of CO in living systems, and investigate their potential applications in the disease treatment using in vivo models. A series of CO-releasing molecules will be engineering by using 3-hydroxyflavone as the parent molecule, and through the rational introduction of another fluorophore and other positioning groups, biologically active species-mediated groups. The project has a solid research basis and implementation environment, as well as demonstrates good feasibility. The implementation of the project is envisaged to develop a variety of metal-free CO-releasing molecules that integrate functions of controlled release, fluorescence imaging, positioning and treatment, thus providing innovative solutions for the safe and effective delivery of CO and CO gas therapy in vivo.