棉铃虫硫酸酯酶与其修饰因子SUMF1解毒寄主植物硫苷分子机制

植物抗虫防御物与昆虫的反防御机制是一场长期的军备竞赛，一方的进化更新会被另一方的相应演化所抵消。目前，参与棉铃虫寄主植物硫苷解毒的功能基因和分子机制还不明确。针对我国新疆地区经济作物重大害虫棉铃虫寄主选择适应性强、为害范围广和农药污染严重等突出问题，围绕植物抗虫防御物与害虫相互作用及其机制这一科学问题，本研究拟解析棉铃虫硫酸酯酶（SULFs）与其修饰因子（SUMF1）解毒寄主植物硫苷分子机制，旨在为棉铃虫绿色防控提出新思路。本项目前期从棉铃虫基因组获得8个SULFs和1个SUMF1基因，并已验证其在棉铃虫中肠的表达。基于此拟研究：1）棉铃虫SULFs与SUMF1基因结构与表达模式；2）SULFs与SUMF1基因表达对含硫苷植物的响应；3）SULFs与SUMF1在解毒硫苷中的作用及调控机制。本研究揭示的SULFs与SUMF1功能和调控机制可为开发防治棉铃虫新农药和针对性治理措施提供分子靶标。

Plant anti-insect defensive and insect anti-defense mechanisms are a long-term arms race, with an evolutionary innovation on one side being countered by a corresponding advance on the other. At present, the functional genes and molecular mechanisms involved in detoxification of the host plant of cotton bollworm are not yet clear. Because of this latter concern of host adaptability, more data is needed regarding the mechanisms of interaction between insect-resistant secondary metabolites and pests. There is increasing concern for the outstanding problems caused by the cotton bollworm, Helicoverpa armigera, which is a major pest of economic crops in Xinjiang, China. These problems include a wide range of crop damage, pesticide pollution, and strong host adaptability. The aim of this study is to analyze the molecular mechanism of sulfatase (SULFs) and its modification factor (SUMF1) in the cotton bollworm in the detoxification of host plant glucosinolates, aiming to propose a new idea for the green control of cotton bollworm.In the early stage of the project, 8 SULFs and 1 SUMF1 genes were obtained from the cotton bollworm genome, and the expression of this series of genes in the midgut of the cotton bollworm was verified. Therefore, this project intends to focus on: 1) the structure and expression pattern of SULFs and SUMF1 genes in the cotton bollworm; 2) the response of SULFs and SUMF1 gene expression to glucosinolate plants; 3) the role and regulation mechanism of cotton bollworm SUMF1 and SULFs in the detoxification of glucosinolates. The research reveals the function and regulation mechanism of SULFs and SUMF1 to provide molecular targets for the development of new pesticides and targeted control measures.