野大豆（Glycine soja Sieb. et Zucc）抵御低磷胁迫的分子机制研究

土壤缺磷是限制我国大豆生产的重要因素。优质野生植物资源的有效开发和利用是培育优质、多抗型农作物新品种的重要途径。优异野大豆种质资源抵御低磷胁迫的生理及分子机制研究将为耐低磷型栽培大豆新品种的选育提供重要的理论基础。本课题拟在前期已获得耐低磷型野大豆材料及对其形态和生理特性研究的基础上，以不同磷效率型野大豆和栽培大豆为实验材料，通过对低磷条件下实验材料幼苗不同器官中离子组学、代谢组学、转录组学及其相互关系的测试与分析，比较研究磷高效野大豆、普通野大豆、栽培大豆之间矿质营养、小分子物质代谢及转录水平的差异；结合代谢物功能分析、代谢途径富集、分子互作的系统研究，全面解析生物小分子功能及其营养和分子调控之间的关联性，从而揭示磷高效型野大豆抵御低磷胁迫的内在分子机制。本研究将为野大豆种质资源的有效利用及磷高效型栽培大豆新品种的选育奠定理论基础，为植物资源评价及鉴定研究提供新思路新方法。

Phosphorus deficiency in soil is an important factor limiting soybean production in China. The effective development and utilization of high quality wild plant resources is an important way to cultivate new varieties of high quality and multi-resistance crops. The studies of physiological and molecular mechanisms of excellent wild soybean germplasm resources against low phosphorus stress will provide an important theoretical basis for breeding new varieties of low phosphorus tolerant cultivated soybean. Based on the previous study of low-phosphorus-tolerant wild soybean materials and their morphological and physiological characteristics, and takingdifferent phosphorus-efficient wild soybean and cultivated soybean as experimental materials, the differences of mineral nutrition, small molecule metabolism and transcription levels among high-efficiency wild soybean, common wild soybean and cultivated soybean were compared by testing and analyzing the ionomics, metabolomics, transcriptome and their interrelation in different organs of seedlings under low phosphorus conditions. Combined with the systematic study of metabolite function analysis, metabolic pathway enrichment and molecular interaction, the relationship between biological small molecular function and its nutrition and molecular regulation is comprehensively analyzed, so as to reveal the intrinsic molecular mechanism of phosphorus efficient wild soybean against low phosphorus stress. This study will lay a theoretical foundation for the effective utilization of wild soybean germplasm resources and breeding of new varieties of phosphorus-efficient cultivated soybean, and provide new ideas and methods for the evaluation and identification of plant resources.