基于抗细菌生物膜活性药源开发的滇西北高寒特境黄芪属植物根际微生物研究

细菌生物膜，亦称菌膜，是造成病原菌耐药性增强和持续感染的主要因素。而目前尚无针对抗菌膜的特效药物。特境植物根际微生物可产生大量具有提高宿主免疫功能的活性成分，极具抗菌膜药源开发潜力。我国滇川藏交界高寒地区为中国黄芪属植物主要分布区域之一，分布有众多应用价值较高、但认识度与资源储量均很低的黄芪属植物，其相关微生物资源亟待开发利用。本项目拟在团队前期工作基础之上，综合采用宏基因组高通量测序、UPLC-MS、光学显微成像技术和生物组学等多种手段充分挖掘滇西北特境黄芪属植物根际微生物的抗菌膜药源潜力，以期得到高效抗菌膜活性菌株及作用机制明确的优良抗菌膜活性化合物，并整体认知抗菌膜活性菌株的物种群落分布与宿主植物及其生态环境的化学生态学关系。本项目将抗菌膜活性药源开发拓展至目前研究较少的滇西北高寒植物来源的微生物，对滇西北特境特色珍稀药用植物及其微生物资源的开发利用与保护具有重要的现实意义。

Bacterial biofilm (also known as biofilm) is a major factor in causing bacterial resistance and nosocomial recurrent infections. But up to now there are still no effective anti-biofilm drugs. Rhizosphere microorganisms of some plants in special environment can produce large quantities of active components which can improve the host immunity, and are thought to be a medicinal resource with great anti-biofilm potential. The border area of Yunnan, Sichuan and Tibet in southwest China is the main distribution area of the Astragalus plants in China. There are many plants of Astragalus with high application value, but low recognition and resource reserves. It is necessary and urgent to systematically study microbes of these Astragalus plants in special environment. Based on the previous work, this study intends to exploit anti-biofilm medicinal resources of microbes derived from the Astragalus plants in special environment of northwest Yunnan through a variety of chemical and biological research techniques including metagenomic high-throughput sequencing technology, ultra-performance liquid chromatography- quadrupole time-of-flight mass spectrometry, optical microscopic imaging technique and biological omics, hoping to get highly effective anti-biofilm microbial strains and compounds with excellent anti-biofilm activity and precise mechanism of anti-biofilm effect. This work will lead to the overall awareness of correlation between host plants or ecological environment and community composition of active microorganisms. This project broadens the research field of anti-biofilm resource, and focuses on the exploitation of microbes in high-cold ecosystem of northwest Yunnan, which is significant for rational utilization and protection of characteristic and rare plants and associated microorganisms in special environment of northwest Yunnan.