过敏诱导反应OsHIR3蛋白抵御水稻条纹病毒侵染的分子机制研究

水稻条纹病毒引起的水稻条纹叶枯病目前仍是我国水稻生产的重要病毒病害之一，需引起我们高度重视。过敏诱导反应HIR基因家族与过敏反应（HR）密切相关，参与植物对病原物的防御反应。尽管HIR蛋白参与许多生物学过程，但其抗病原物侵染的分子基础尚不明确，我们拟对此开展工作。项目前期工作发现，OsHIR3基因参与植物抗RSV侵染过程；OsHIR3与RSV NS3蛋白在细胞质膜存在共定位；酵母双杂交系统显示OsHIR3与RSV NS3蛋白在体内存在相互作用，暗示OsHIR3蛋白可能与RSV NS3蛋白之间具有相关性。.本项目拟以OsHIR3蛋白为切入点，从分子层面解析寄主与病毒的互作机制：明确OsHIR3与RSV NS3蛋白的互作；鉴定其互作的关键结构域；明确互作对RSV NS3功能的影响；构建OsHIR3蛋白在植物抗RSV侵染的分子模型，这对于深入理解水稻针对病毒侵染的调控机制具有一定的理论价值。

Rice stripe disease, resulted from Rice stripe virus (RSV), is still one of the important rice viral diseases in China. The hypersensitive induced reaction(HIR) gene family is associated with hypersensitive response (HR), participating in plant defense against pathogens. Although the role of HIR proteins involved in many biological processes have been well studied, little is known about the molecular basis of its disease resistance, so we plan to work on it. In our previous work, we found that OsHIR3 gene was involved in plant resistance against RSV infection; OsHIR3 co-localized with RSV NS3 in the plasma membrane; OsHIR3 interacted with RSV NS3 protein in vivo in yeast two hybrid system, suggesting that OsHIR3 protein might be related to RSV NS3 protein..In this project, OsHIR3 protein will be used as the entry point to analyze the interaction mechanism between host and virus from the molecular level: to clarify the interaction between OsHIR3 and RSV NS3 protein; to identify the key domain of the interaction; to explore the effect of interaction on RSV NS3 function; to construct the molecular model of OsHIR3 protein involved in plants against RSV infection, which has certain theoretical value for further understanding the regulatory mechanism of rice against virus infection.