水稻LONG PANICLE1基因调控穗长的分子机制研究

挖掘水稻长穗优异等位基因并阐明其分子机制有助于培育长穗直立粳稻品种从而提高籽粒产量。申请人前期利用水稻自然群体及C堡和秀水79构建的重组自交系共同定位到一个穗长主效位点，并成功克隆到一个全新的长穗基因LONG PANICLE1，但其分子机制尚不明确。本项目拟进一步验证LONG PANICLE1的功能，通过实时荧光定量、启动子活性分析及原位杂交方法确定LONG PANICLE1在幼穗中的精细时空表达图谱；通过酵母双杂交、荧光双分子互补、体外Pull down实验挖掘并验证与LONG PANICLE1互作的蛋白；结合生物信息学方法解析该基因调控水稻穗长的分子机理。再利用400份水稻种质分析LONG PANICLE1的等位基因多态性及其在群体内的演变规律。本项目的成功实施将为粳稻短直立穗的遗传改良提供理论参考和遗传依据。

Mining elite alleles of long panicle in rice and elucidating its molecular mechanism has great significance to breed long-erect panicle japonica varieties and thus improve grain yield. LONG PANICLE1, as a new gene of panicle length, was successfully cloned by association analysis in natural population and linkage analysis in recombinant inbred lines derived from the cross by C-bao and Xiushui79, but its molecular mechanism was not clear. On this basis, the function of LONG PANICLE1 will be further verified in this project. Fine temporal and spatial expression analysis of LONG PANICLE1 in young panicles will be conducted by real-time fluorescence quantitative analysis, promoter GUS activity analysis and RNA in situ hybridization. Yeast two-hybrid, bimolecular fluorescence complementation assay and pull-down in vitro will be conducted to detect and identify the proteins interacting with LONG PANICLE1.The molecular mechanism of LONG PANICLE1 gene regulating panicle length in rice will be elucidated by the results above combining with bioinformatics. Finally, the evolution of alleles at LONG PANICLE1 locus will be studied by investigating and analyzing the allelic polymorphism in 400 accessions. The successful implementation of this project will provide theoretical reference and genetic basis for genetic improvement of short erect panicle in japonica rice.