BnaFAX6.1在甘蓝型油菜根尖介导木栓质单体运输过程中的分子机制研究

木栓质在调控根系对矿物营养吸收和抵御病害等方面起到重要作用。甘蓝型油菜(B.napus)是重要的油料作物，但是油菜根系木栓质单体跨膜输出过程尚未清晰，这成为营养吸收高效型油菜品种选育的制约因素之一。申请人发现了一类新的膜蛋白家族FAX。油菜体内的FAX家族蛋白BnaFAX6.1定位在质膜上，BnaFAX6.1在根尖的表达量显著高于其它组织，BnaFAX6.1在拟南芥中异源表达可显著提高木栓质的积累量。在此基础上，该项目拟通过针对BnaFAX6.1的亚细胞精细定位、根尖组织表达分析、突变体油菜的木栓质累积特征分析、木栓质单体的运输功能验证实验，以及BnaFAX6.1的上游转录因子筛选与调控机制验证研究等，阐明BnaFAX6.1在油菜根尖运输木栓质单体的分子机制并建立其调控模型，为解析木栓质积累在油菜根系营养吸收中的作用提供分子水平的证据，为未来选育营养高效型油菜品种提供重要依据。

Suberin plays an important role in regulating root uptake of mineral nutrition and response to pathogens. Rapeseed is one of the most important oil crops, but the transport of suberin monomers remains unknown, which has become a bottleneck restricting the molecular breeding of rape varieties with high nutrient uptake efficiency. The applicant’s previous research discovered a new class of membrane protein family FAX. The FAX homologous protein BnaFAX6.1 in rape is located on the plasma membrane. The expression of BnaFAX6.1 at the root apex is significantly higher than other tissues. The expression of BnaFAX6.1 in Arabidopsis can significantly improve the accumulation of suberin amount. On this basis, the proposal intends to perform subcellular localization, tissue expression analysis, mutant phenotype analysis, suberin monomer transport function analysis, and BnaFAX6.1 upstream transcription factor screening and regulatory mechanism validation studies, etc., to clarify the molecular mechanism of BnaFAX6.1 transporting suberin monomers in rapeseed root tips. Providing molecular level evidence to provide an important basis for future breeding of nutritious and efficient rapeseed varieties.