油体膜蛋白基因OLEs在芍药种子油体形成中功能解析

油体是油料植物种子储存油脂的细胞器，其大小、数量和稳定性与种子含油量密切相关。目前，有关牡丹和芍药籽油的积累机制研究侧重于脂肪酸合成及其相关基因的挖掘，而对于贮存油脂的油体研究尚未涉及。油体膜蛋白（OLEs）影响植物油体形成和稳定的重要因素，了解油体膜蛋白基因OLEs生物学功能对揭示植物脂类代谢机制有着重要作用。本研究拟以含油量高低不同的两个‘杭芍’品系为材料，在分析油体组织形态、理化特性的基础上，通过转录组与蛋白组联合分析筛选调控油体形成的OLEs基因，再进一步通过表达模式检测、亚细胞定位分析、烟草和拟南芥突变体转化、体外脂质体融合分析、酵母双杂交筛选OLEs基因互作蛋白来明确油体形成的关键OLEs基因及其生物学功能，解析与OLEs基因互作蛋白的作用关系，从而详尽阐述OLEs在芍药种子油体形成中的功能，为提高芍药种子含油量提供基因资源和理论依据。

Oil body (OB) is a unique oil storage organelle of oilseed plants. The size, number and stability of OB have close relationship with oil content of seed. At present, the study of tree peony and herbaceous peony seed oil accumulation mechanism only focuses on the fatty acid synthesis and digging related genes. However, there is none study involved in OB formation. Oleosins (OLEs) are the main protein of OB, and affect the formation and stability of OB. To understand the biological functions of OLEs can provide an great contribution to reveal the mechanism of plant lipid metabolism. Our study intends to use high and low oil content P. lactifolra 'Hangshao' strains as material. Based on the organization form, physical and chemical properties research of oil body, we are going to screen out the whole OLEs through combination analysis of transcriptome and iTRAQ. Furthermore, expression pattern construction, subcellular localization, gene transformation into tobacco and Arabidopsis mutants, fusion of key genes with liposome in vitro, yeast two hybrid will all be introduced to confirm the key OLEs and their biological functions, to help for understanding of interaction relationship between OLEs and other proteins. The above researches will further illustrate the details of OLEs function during oil body morphogenesis, and provide gene resources and theoretical basis for improving the herbaceous peony seed oil content.