猪繁殖和呼吸综合征疫苗株感染后对DC细胞抗原递呈功能影响的研究

免疫接种理应作为符合我国国情的临床防控猪繁殖和呼吸综合征最有效的手段，但目前疫苗临床保护效力受到免疫后体液免疫和细胞免疫应答水平低下等问题的制约，严重影响着猪场临床防控措施的制定，导致PRRS成为严重影响我国生猪养殖的顽疾。解决这些问题最根本在于理清现有弱毒疫苗免疫后体液（中和抗体）和细胞免疫应答水平低下的原因，继而解析疫苗免疫保护机理，从而有针对性的对疫苗进行改进。本研究以商品化的PRRS疫苗株HuN4-F112和其母源强毒HuN4为病毒模型，以体内主要抗原递呈细胞DC为对象，以DC细胞对病毒的捕获、加工和呈递以及展示抗原效率为主线，通过对病毒关键组分与DC细胞相关分子互作与调控机制的解析，阐明PRRSV疫苗株感染后对DC抗原递呈功能的影响及分子机制，以期从机理上解释疫苗免疫后体液（中和抗体）和细胞免疫应答水平低的临床实际问题，同时为新疫苗的改进提供理论支撑。

Vaccination should be the most effective and suitable means for the control of porcine reproductive and respiratory syndrome(PRRS) in the field. However, there is a huge controversy over the use of the porcine reproductive and respiratory syndrome vaccine, including low level of humoral(neutralizing antibody) and celluullar immune response after immunization and causing dilemma of PRRS control in the field. The key to soving thee problems is to understanding the mechanism of low level of immune response after immunization, further more improve the design of vaccine. In this study, PRRSV wild type strain HuN4 and its attenuated vaccine HuN4-F112 strain were chose as virus model, and the main antigen presenting cells- Dendritic cells(DC) in vivo were targeted . As a main line, antigen processing, presentation and the stable of MHC molecular on cell member were detected and the mechanism of viral protein interactive with the key protein in these route were investigated. The results will provide a new perspective for the answer of low level of humoral(neutralizing antibody) and celluullar immune response after immunization, and give more detail about immune protection mechanism, more over provide a theoretical basis for the development of new vaccines and the prevention of PRRS.