转录因子CitERF106调控云南秋花春熟青柠檬采后褪绿不均的分子机理

云南是我国重要柠檬产区，近年来主推的秋花春熟青柠檬生产新技术有效填补了市场春季鲜果供给的空档。但果实采后极易形成黄绿不均的花斑果，严重影响了果实的外观品质。前期通过对花斑果有色层绿色和褪绿部位及外源赤霉素（GA3）和乙烯利（CEPA）的正负调控下果皮色素含量、激素水平和转录组学分析，发现CitERF106很可能是响应激素信号的关键转录因子，调控果皮色泽不均匀现象，但其具体的作用机制尚待深入研究。本项目拟以“云柠1号”秋花果青柠檬为试材，通过EMSA、酵母双杂及双荧光素酶实验验证CitERF106及互作转录因子对色素相关基因的调控，转化滑皮金柑及山金柑对CitERF106进行功能验证。同时，构建CitERF106启动子-GUS载体并转化柑橘愈伤组织，验证CitERF106对GA3和乙烯的响应，阐明CitERF106对青柠檬褪绿不均的调控机制，为云南青柠檬采后贮藏保鲜和技术创新提供科学依据。

Yunnan is an important lemon producing area in China. In recent years, the new production technology of spring-ripening green lemon has effectively filled the supply gap of fresh fruit in spring. However, green lemon is easy to produce uneven yellow and green fruit after harvest, which seriously affected the appearance quality of the fruit. The former study about the analysis of the pigment, hormone treatment and transcriptome indicated that CitERF106 was probably the key transcription factors regulating the pigment metabolism of spring-ripening green lemon, but the specific mechanism of action still needs to be further studied. The project plans to use "Yunnnig number 1" green lemon as the research material. Based on the former study, the regulation of CitERF106 on the pigment metabolism related genes will be analyze by EMSA, Yeast-2-Hybrid and dual luciferase assay. And the function of CitERF106 will be confirmed by evaluating the pigment metabolism of the CitERF106 transgenic Hua’pi kumquat and Hongkong kumquat. Moreover, the vector fusing the promoter sequence of CitERF106 and GUS sequence will also be transformed to citrus callus to check the response of CitERF106 promoter to the hormone treatment. The present research will through analyze the transcriptional regulation of CitERF106 on the uneven chlorosis, which will supply scientific evidences to the theoretical and technological innovation for storage and preservation of Yunnan spring-ripening green lemon.