基于食物成分相互作用及代谢组学技术构建咖啡酰奎宁酸高效生物利用食物运载体系及其机理研究

咖啡酰奎宁酸（CQAs）具较强的抗菌、抗病毒等活性，是食物中重要的功能成分。研究组前期调控悬浮培养金银花细胞的次生代谢使CQAs积累达到9.6%（干重）以上。本项目针对其生物利用率不高现状，拟利用代谢组学技术和食物成分相互作用原理构建咖啡酰奎宁酸高效生物利用食物运载体系并解析其机理。利用液质的代谢组学技术分析CQAs在体内的代谢轮廓、代谢标志物并获得生物利用率科学计量方法；将食物基质逐步细分类，采用单因素及响应面设计实验筛选促进CQAs生物利用的食物基质；利用光谱、色谱、质谱技术分析食物基质与功能成分生物利用率的构-效关系、量-效关系；优化条件构建CQAs高效食物运载体系；从体系中CQAs的溶解性、释放度、跨细胞膜转运特性阐明作用机理。研究结果将为CQAs高效生物利用食物运载体系构建和其机理解析提供理论依据，并为后续CQAs健康产品开发和其功能在体内充分发挥提供潜在的技术支撑。

Caffeoylquinic acid (CQAs) has strong biological activities such as antibacterial, antiviral, and is an important functional component in food. From the results of our group′s previous study, the accumulation of CQAs in cells was improved and reached to 9.6% (dry weight) by regulating the secondary metabolism of suspension cultured Lonicera japonica cells. This project aims at construct a caffeoquinic acid high-efficiency food delivery system and analysis its mechanism via using metabolomics and food ingredients interaction principles to overcome its low bioavailability. The LC-MS metabolomics will be used to analyze the metabolic profile and metabolic markers of CQAs in the body and to obtain a scientific measurement method of bioavailability. The food substrate will be gradually classified according to structural characteristics. The food matrix which significantly affect the bioavailability of CQAs will be screen by the single-factor and response surface design experiments. The structure-effect relationship and dose-effect relationship between functional components bioavailability and food matrix will be analyzed using spectrum, chromatography and mass spectrometry. a CQAs efficient food delivery system will be constructed by optimizing conditions, and the mechanism of action will be elucidated via analysis of the solubility, release degree, and transcellular membrane transport characteristics of CQAs in the system. The research results will provide a theoretical basis for the construction of CQAs efficient bioavailable food delivery system and its mechanism interpreting. It also provides a potential technical support on subsequent developments of CQAs health product and full applications in the body .