新型低聚类有机磷阻燃剂在斑马鱼体内的富集行为及毒性效应研究

阻燃剂在人们的日常生活中对保障人员和财产安全起到了重要作用，全球对阻燃剂的需求量越来越大。为了弥补溴代阻燃剂及早期应用最广泛的几种氯代有机磷阻燃剂(Cl-OPFRs)被禁用带来的市场空缺，低聚类OPFRs因其优良的性能受到广泛青睐，其生产和使用量大幅上升，其环境行为应引起重视。目前关于低聚类OPFRs在水环境中的行为和毒性效应的研究非常缺乏，难以满足对其生态风险进行科学评估的需求。低聚类OPFRs在鱼体内是否产生累积效应，主要富集在哪些组织中，通过何种途径清除出体外，是否具有生物毒性，具体的毒性效应及分子机制等均不清楚。本项目拟以斑马鱼为受试生物，研究两种典型低聚类OPFRs在鱼体内的富集和清除动力学过程，揭示其在生物体内的分配行为；筛查其主要毒性效应并阐明作用机制。该研究为全面评价用低聚类OPFRs作为替代品的安全性，为制定合理的替代方案提供科学依据。

Flame retardants have played an important role in ensuring the safety of people and property in daily lives. The global demand for flame retardants is increasing. In order to make up for the market vacancy caused by the ban of some brominated flame retardants and chlorinated organophosphorus flame retardants (Cl-OPFRs), oligomeric OPFRs received a widespread favor. Because their usage has risen sharply, their environmental behavior should be paid more attention. However, the presence of studies on the behavior and toxic effects of these two oligomeric OPFRs in the water environment are rare. “Whether the oligomeric OPFRs could bioaccumulate in the fish? Which is the main organization the oligomeric OPFRs will enriched in? How do them clean up from fish? What are the toxic effects and molecular mechanism of these oligomeric OPFRs?” All these questions are unclear. The lack of data hinders the environmental risk assessments of these oligomeric OPFRs. Therefore, this study intends to use zebrafish as the target organism to study: 1) the bioaccumulation and clearance of two oligomeric OPFRs in fish; 2) screening the main toxic effects of the two oligomeric OPFRs; 3) exploring the toxic mechanism of oligomeric OPFRs in fish from three aspects: individual, tissue and molecular mechanism. This study will provide a scientific basis for comprehensively evaluating the safety of using the two oligomeric OPFRs as substitutes and formulating reasonable alternatives.