糖复康通过调节肠道菌群介导的APN-AMPK-BCAA信号通路对2型糖尿病发挥治疗作用

T2DM作为全球重点关注疾病，中药复方在其治疗中展现出良好的疗效，但由于缺乏合理的研究方法，中药复方作用机制研究陷入瓶颈。研究表明，肠道菌群紊乱影响BCAA合成、运输及分解代谢，导致T2DM的发生发展，而APN-AMPK是调控BCAA分解代谢的上游通路。前期研究显示，在T2DM中APN-AMPK-BCAA通路发生改变，糖复康通过APN-AMPK通路对T2DM发挥治疗作用。综上，本项目将重点研究“糖复康通过肠道菌群介导的APN-AMPK-BCAA信号通路对2型糖尿病发挥治疗作用”。具体拟开展以下内容：16s rDNA测序、粪菌移植实验，验证“肠道菌群是糖复康干预T2DM的关键靶点”。靶向代谢组学、分子生物学实验，验证“糖复康通过调节肠道菌群介导的APN-AMPK-BCAA信号通路对T2DM发挥治疗作用”，此研究结果将为中药复方作用机制研究提供新的思路。

T2DM is a global disease of focus, and TCM compounds show good efficacy in the treatment of T2DM. However, due to the lack of reasonable research methods, the research on the mechanism of action of TCM compounds is in a bottleneck. Studies have shown that intestinal flora disorders affect the synthesis, transportation and catabolism of BCAA, leading to the development of T2DM. APN-AMPK is an upstream pathway that regulates the catabolism of BCAA in host. Previous studies have shown that the APN-AMPK-BCAA pathway is changed in T2DM, and Tang-Fu-Kang plays a therapeutic role on T2DM through the APN-AMPK pathway. According to the above, this project will focus on the study of "the therapeutic effect of Tang-Fu-Kang on T2DM through the APN-AMPK-BCAA signaling pathway mediated by intestinal flora". Specifically, the following contents are proposed: 16s rDNA sequencing and fecal bacteria transplantation experiments to verify that "the intestinal flora is a key target for Tang-Fu-Kang intervention in T2DM"..Targeted metabolomics and molecular biology experiments verified that " Tang-Fu-Kang plays a therapeutic role on T2DM by regulating the APN-AMPK-BCAA signaling pathway mediated by intestinal flora", which will provide a new idea for studying the mechanism of action of TCM compounds.