快速生长生态型蓝藻Synechococcus elongatus 2973光合膜高效转化光能的超分子结构基础

快速生长生态型蓝藻Synechococcus elongatus 2973（Syn2973）光合效率可达同种模式蓝藻Syn7942的两倍，高效光合作用能够更加高效转化利用光能。目前有关Syn2973高效光合作用的研究主要集中于遗传、生理、生化层面。光合光能转化由光合膜上多种光合复合物协作完成，然而Syn2973光合膜高效转化光能的超分子结构基础尚未被阐明。本项目将首先比较不同光强下Syn2973与Syn7942的光合效率、光合复合物含量及性能，揭示Syn2973行使高效光合作用的关键光合复合物；然后利用原子力显微镜技术比较不同光强下两者的光合膜超分子结构，揭示Syn2973光合复合物的特殊结构状态、排布模式及相互作用；最后结合生理特性及超分子结构，阐明Syn2973光合膜高效转化光能的超分子结构基础。该研究将加深对蓝藻高效利用光能的认识，为调控植物光合效率及构建高效人工光合膜提供理论基础。

The photosynthetic efficiency of a fast-growing cyanobacterium ecotype Synechococcus elongatus 2973 (Syn2973) can reach twice that of the type species of congeneric cyanobacteria, Synechococcus elongatus 7942 (Syn7942). Efficient photosynthesis can convert and utilize light energy more efficiently. Many studies about the efficient photosynthesis of Syn2973 have been performed from the perspective of genetics, physiology, and biochemistry. Photosynthetic light energy conversion is conducted by the collaboration of various photosynthetic complexes in photosynthetic membrane. However, the supramolecular architecture basis for efficient conversion of light energy by photosynthetic membrane in Syn2973 remains unknown. In this project, Syn2973 and Syn7942 will be grown under different light intensities and used for further experiments. First, we will analysis the differences of their photosynthetic efficiency, and the content and capacity of their photosynthetic complexes to reveal the key photosynthetic complex for efficient photosynthesis of Syn2973. Then, we will investigate the supramolecular structures of their photosynthetic membranes using atomic force microscopy to reveal the special configuration, arrangement, and interaction of photosynthetic complexes of Syn2973. At last, comprehensively analyze the physiological properties and the supramolecular structures of photosynthetic membranes to elucidate the supramolecular architecture basis for efficient conversion of light energy by photosynthetic membrane in Syn2973. This work will deepen our understanding of efficient utilization of light energy in cyanobacteria and offer a theoretical underpinning for regulating the photosynthetic efficiency of plants and constructing efficient artificial photosynthetic membranes.