彝药美洲大蠊多肽原位驻留长效递释系统的构建及抗溃疡性结肠炎作用研究

本项目在课题组数十年对药用昆虫美洲大蠊系列研究的基础上，根据体内外药效活性指导，使用新工艺从美洲大蠊中精制了对溃疡性结肠炎有显著治疗作用的活性部位PA。前期研究显示：PA以多肽类物质为主要成分且通过JAK/STAT3/SOCS3通路调节肠黏膜免疫，促进修复相关的细胞因子表达，但仍存在PA与结肠作用时间短、疗效不稳定的问题；为进一步延长PA在结肠内的驻留时间、提高药物疗效及降低用药频率，本研究提出了PA纳米粒原位驻留长效缓释及其调控溃疡性结肠炎黏膜免疫及修复的药物递释系统。本研究结果预期可为PA应用更高效的结肠靶向纳米制剂技术和提高临床的UC治疗效果提供理论基础，为更好的应用开发云南彝族药物提供科学依据。

On the basis of our continuous series researches for decades on the medicinal insect Periplaneta americana, anew bioactive extract PA with notable therapeutic effects towards ulcerative colitis (UC) was obtained using the new purification process andthe guidance of in vitro and in vivo bioactivity assays. Previous studies explored that PA is mainly composed of polypeptides, and which regulated intestinal mucosal immunity through JAK/STAT3/SOCS3 pathway to promote the expression of repair-related cytokines. But there were still problems that PA did short duration of action in the colon, and its effect was not stable. In order to further prolong the residence time of PA in colon, improve its curative effect and reduce its frequency. In this study, the Drug delivery system that long-term sustained release of PA nanoparticles in situ and its regulation on mucosal immunity and repair of ulcerative colitis are proposed. The results of this study are expected to provide a theoretical basis for the applications of more efficient pharmaceutical technology of colonic targeting preparations towards the bioactive extract and improving the clinical efficacy of PA in the treatment of UC, as well as to provide scientific basis for better development of Yunnan's Yi nationality characteristic insect medicine.