甜橙中与柑橘黄龙病相关NBS-LRR类抗病基因的挖掘及功能分析

柑橘黄龙病（HLB）是柑橘产业上最大的危害之一，目前无有效防治手段。抗柑橘黄龙病品种的培育是大家努力的方向，需要深入理解柑橘免疫系统与柑橘黄龙病菌（Candidatus liberibacter）之间的互作。目前关于柑橘中与HLB相关的抗病基因只有零星报道，且没有关于NBS-LRR类抗病基因相关的研究。NBS-LRR类抗病基因是植物免疫系统ETI中的关键基因，有效帮助植物抵御病原的侵染。本项目拟结合比较基因组学与系统发生学方法挖掘甜橙中所有NBS-LRR类抗病基因，揭示柑橘中NBS-LRR类抗病基因的多样性。在此基础上，本项目拟利用转录组测序技术筛选出与HLB相关的抗病基因，结合过表达实验进行功能验证，解析抗病基因的功能。本项目的开展将揭示甜橙中与HLB相关抗病基因的多样性，为柑橘抗HLB机制，研究HLB致病机制的研究打好基础，对抗HLB品种的培育提供理论基础，具有重大的理论意义和应用前景

Citrus huanglongbing (HLB) is one of the biggest hazards in the citrus industry and no effective control method currently. The cultivation of varieties resistant to citrus HLB is the direction of everyone's efforts, which is necessary to understand the interaction between citrus immune system and Candidatus liberibacter. Only anecdotal reports on HLB-related disease resistance genes in citrus, and no research related to NBS-LRR resistance genes has been reported. Plant NBS-LRR resistance genes are the key genes in the immune system ETI, which help plant resist pathogen infection. This project intends to combine comparative genomics and phylogenetic methods to identify all NBS-LRR disease resistance genes in sweet oranges and reveal the diversity of NBS-LRR disease resistance genes in citrus. On this basis, we will use transcriptome-sequencing technology to screen out disease-resistant genes related to HLB, combine with overexpression experiments for functional verification, and analyze the function of resistant genes. The development of this project will reveal the diversity of HLB-related resistance genes in sweet oranges, reveal a solid foundation for the research on the anti-HLB mechanism of citrus, study the pathogenesis of HLB, and provide a theoretical basis for the cultivation of HLB varieties. It has great theoretical significance and application prospects.