GnIH在卵巢内调控马岗鹅卵泡发育的分子机制

促性腺素抑制激素（GnIH）是禽类下丘脑关键生殖调控因子，能直接或间接通过GnRH抑制垂体促性腺激素分泌；然有研究提示，GnIH还能直接在性腺发挥调控作用，但对其机制知之甚少。项目研究马岗鹅：①卵泡发育过程中大白卵泡、小黄卵泡及大黄卵泡颗粒层和膜层中GnIH、GnIHR、FSHR、LHR、P450arom、StAR、INH、ACT表达变化；②光照调控繁殖季节性转换过程中，结合主动免疫GnIH，对产蛋、卵泡发育、以上基因在卵巢内表达及血液FSH、LH、PRL、P4、E2、INH水平的影响；③不同繁殖期（休产、产蛋、就巢），卵泡发育、以上基因在卵巢内的表达及血液FSH、LH、PRL、P4、E2、INH水平；④外源GnIH或抗GnIH抗体对颗粒细胞和膜细胞增殖、凋亡和以上基因表达及P4、E2、INH分泌的影响；揭示GnIH在卵巢内调控马岗鹅卵泡发育的分子机制，为完善禽类生殖调控理论提供科学依据。

As a key reproductive regulator in hypothalamus of avian, Gonadotropin inhibitory hormone (GnIH) can inhibit the gonadotropin secretion directly or indirectly through GnRH. However, previous studies have shown that GnIH could directly regulate follicular development in gonads, but little is known about its mechanism. Taking Magang goose as the object, this project would study: ①The gene expression patterns of GnIH、GnIHR、FSHR、LHR、P450arom、StAR、INH、ACT in the large white follicles (LWF), small yellow follicles(SYF) and the granulosa cells and theca layer of the large yellow follicles (LYF) during the follicular development. ②The egg laying, the follicular development, the change rule of the above genes in ovary and the levels of FSH, LH, PRL, P4, E2 and INH in blood during the seasonal transformation process of reproductive activity by artificial lighting, and the effects of active immunization with GnIH. ③The patterns of follicular development, expression of the above genes in the ovary and the blood levels of FSH, LH, PRL, P4, E2 and INH in different reproductive stages (no-breeding, laying and incubation). ④The effects of exogenous GnIH or anti-GnIH antibody on proliferation, apoptosis of granulosa cell and theca cell, and expression of the above genes and secretion of P4, E2 and INH. Those above results will reveal the molecular regulation mechanism of GnIH on follicular development in ovary of Magang goose, and provide scientific evidences for perfecting the reproductive control theory of birds.