包皮内板菌群激活CD4 T细胞引起自身免疫性CP/CPPS的机制研究

慢性前列腺炎/慢性骨盆疼痛综合征（CP/CPPS）是50岁以下男性最常见的泌尿系统疾患，其特征是会阴及骨盆区域的疼痛、尿频和性功能障碍。自身免疫诱发CP/CPPS的理论越来越受到重视。我们的前期研究发现包皮过长人群中CPPS患者和正常人群的包皮内板上的菌群存在明显的差异，并且CPPS患者的包皮内板菌群可以诱发NOD小鼠形成自身免疫性前列腺炎的相关症状，在这个过程中CD4 T细胞家族中的Th1、Th17等细胞有着重要的作用。包皮内板菌群的差异可以导致CD4 T细胞的激活和IL17、IFN-γ的分泌，在前列腺中形成自身免疫引发慢性炎症，进而引起症状发生。本课题拟用免疫表型分析、流式细胞术和16s-rRNA高通量测序等方法进行研究，探索包皮菌群通过激活CD4 T细胞在前列腺中分泌的IL17、IFN-γ致使CPPS症状发生的过程。这一课题对阐明包皮菌群对自身免疫性CPPS的发生机制有着重要意义。

Chronic prostatitis/chronic pelvic pain syndrome CP/CPPS is the most common urinary system disorder in men under 50 years of age. The theory that autoimmune theory induces CP/CPPS is getting more and more attention. In our previous study, there was a significant difference in the flora of the foreskin of CPPS patients and non-CPPS normals in the prepuce population, and the foreskin flora of CPPS patients can induce autoimmune prostatitis in NOD mice. In combination with literature and previous experiments, we found that Th1, Th17 cells in the CD4 T cell family and the immune factors secreted by them play an important role in the autoimmune. Differences in the bacterial flora of the inner foreskin can lead to the activation of CD4 T cells and the secretion of IL17 and IFN-γ, and the formation of autoimmunity in the prostate leads to chronic inflammation, which then causes pain in the perineal and pelvic areas. In this study, immunophenotypic analysis, flow cytometry, 16S-rRNA high-throughput sequencing and other methods were used to explore the process of the occurrence of CPPS symptoms caused by the foreskin flora through the activation of IL17 and IFN-γ secreted by CD4 T cells in the prostate. This study is of great significance to elucidate the mechanism of the occurrence of puce bacteria on autoimmune CPPS.