大豆花期基因qFT2的精细定位及功能解析

前期利用遗传背景差异较大的中国栽培大豆品种绥农10号和日本栽培大豆品种Toyomusume杂交构建群体。利用F2代群体和Illumina SoySNP8k iSelect BeadChip 芯片构建连锁图谱、F3代群体和SSR标记构建连锁图谱、及F4代群体和2号染色体标记连锁分析，定位到调控花期新位点qFT2，位于Chr02：13.62-14.76Mb。本项目拟在前期研究基础上，精细定位并解析该基因功能：1、利用定位区域分子标记，筛选栽培大豆杂交后代群体重组体，结合野生大豆定位分析，精细定位该基因；2、根据基因功能注释及序列多态性确定候选基因；3、构建候选基因超表达载体和CRISPR基因敲除载体并转化到受体品种，初步验证基因功能；4、分析花期基因转录表达模式及GFP 融合蛋白亚细胞定位；5、开发花期基因联锁标记及在育种中应用。研究对丰富大豆花期基因及培育大豆新品种具有一定科学和实用价值。

In the previous study, We developed a population of Chinese cultivar ( Suinong 10 ) × Japanese cultivar ( Toyomusume ) to map novel quantitative trait locus(QTL) for flowering time. A genetic linkage map of a F2 population was constructed using single nucleotide polymorphism (SNP) markers using lllumina SoySNP8K iSelect BeadChip, and a novel locus was mapped in Chr02：13.62-14.76Mb. Using other sets of F2 populations and their derived progenies, the existence of the novel QTL was verified. Based on previous studies, this research tries to carry out the followed studies in depth: The first, we want to select recombinants by SNP markers to map the gene deeply. The second, we try to forecast the candidate genes according functional annotation and sequence polymorphism. The third, we want to prove the genes' function by constructing vectors and genetic transformation. The fourth, we want to analyze the transcription expression pattern and subcellular localization of GFP fusion protein. The fifth, we want to develop and use linkage markers of flowering time gene in breeding. Our study is scientifically and practically important to detect new soybean flowering time gene, and breed soybean varieties.