StOFP20调控马铃薯薯形的分子机理研究

马铃薯是世界上最重要的粮食作物之一，其薯形直接影响马铃薯的外观品质和经济价值。Ro是控制薯形的主效基因， OVATE family proteins (OFPs) 家族的StOFP20基因与薯形共分离，可能是Ro位点的编码基因。申请人前期获得了薯形的二倍体分离群体，通过精细定位发现薯形也是由Ro位点控制的，敲除StOFP20导致薯形由圆形变为椭圆形，表明StOFP20是控制薯形的基因。在此基础上，本项目拟采用亚细胞定位和原位杂交等方法，明确StOFP20蛋白的亚细胞定位及表达时空模式；结合细胞学观察，探究不同薯形在细胞大小、数量或者形态方面的差异；利用敲除和过表达StOFP20植株的转录组数据及激素响应实验，分析StOFP20参与的激素途径；进一步利用酵母体系和植物体系，筛选和验证互作蛋白。研究结果将探明StOFP20调控薯形的分子作用机理，为培育优质的马铃薯品种提供分子标记和理论依据。

Potato （Solanum tuberosum）is one of the most important food crops of the world，and the tuber shape directly impacts the appearance quality and economic value. Ro has been revealed to be a major locus controlling tuber shape. Previous studies had demonstrated that OVATE family protein 20 (StOFP20) was co-segregated with tuber shapes, and likely underlied the Ro locus. We obtained a population segregating for tuber shape, and fine-mapping delimited the causative locus to the same interval as that for Ro. Disabling of StOFP20 led to an altered tuber shape from round to oval shape, which proved the function of StOFP20 and lay a solid foundation for mechanism study. This project will : (1)utilize the methods of subcellular localization and in situ hybridization to reveal the subcellular localization and expression pattern of StOFP20; (2) understand the cellular difference in cell size, number or shape between round and oval tubers based on the histological analysis; (3) dissect the hormonal pathways that StOFP20 is involved on the basis of transcriptome analysis and hormonal response experiments; (4) screen and corroborate the interacting proteins with the yeast and plant experiment systems. The results will unravel the molecular mechanism of StOFP20 controlling tuber shape, which will provide molecular markers and new knowledge for developing elite potato varieties.