CD44蛋白参与口蹄疫病毒巨胞饮方式入侵细胞的机制

口蹄疫病毒（FMDV）作为严重影响畜牧业健康发展的动物病毒，其入侵细胞的途径及具体机制是当前追踪的热点，对研制靶向疫苗实现早期预防具有指导意义。我们前期证实FMDV可利用巨胞饮途径入侵宿主细胞，并通过蛋白组学及表达干扰等发现，膜蛋白CD44作为与受体酪氨酸激酶（RTKs）有直接关系的细胞粘附分子（CAMs），与FMDV的感染早期有关。因此，本研究将继续追踪CD44蛋白是否作为FMDV巨胞饮途径的重要分子参与启动和/或调控FMDV细胞入侵及具体机制？分别在细胞和活体水平探究CD44与RTKs活化、巨胞饮启动的关系，挖掘巨胞饮中可能与CD44蛋白相互作用的受体和辅助因子，追踪CD44与受体/辅助因子共同参与巨胞饮下游激酶、GTP酶信号通路发挥对病毒感染的调控机制。不仅为FMDV感染致病机制提供研究数据，也为高效靶向疫苗的分子设计及广谱抗病毒制剂的靶位点选择提供依据。

Foot-and-mouth disease virus (FMDV), as an animal virus, seriously affects the healthy development of animal husbandry. Its endocytosis and specific mechanism has become a hot topic in virology research, which is of significance to the development of targeted vaccines for rapid prevention. We previously confirmed that FMDV can invade host cells by using the macropinocytosis pathway. By proteomics and gene interference, we further found that membrane protein CD44, as a cell adhesion molecule (CAMs) directly related to receptor tyrosine kinases (RTKs), is related to the early stage of FMDV infection. Therefore, this study will continue to trace whether CD44 proteins, as important molecules in the FMDV macropinocytosis pathway, are involved in initiating and/or regulating FMDV cellular endocytosis and what is specific mechanisms. The relationship between CD44 and RTKs activation in macropinocytosis, and the receptors and co-factors that may interact with CD44 proteins in macropinocytosis, and downstream kinase and GTPase signaling pathways of macropinocytosis in which receptors/co-factors together with CD44 jointly participate and play a regulatory mechanism on virus infection, were investigated at the cellular and in vivo levels, respectively. It not only provides the research data for the pathogenic mechanism of FMDV infection, but also provides the basis for the molecular design of effective targeted vaccines and the selection of the target sites of broad-spectrum antiviral agents.