膜蛋白COLD6参与水稻低温感知的分子机理

植物细胞低温感知器是启动低温应答和激活防御反应的关键环节，但有关该过程的分子机制却知之甚少。我们的前期研究表明，膜蛋白COLD1参与了水稻低温信号的感知，并激活下游耐寒防御反应。近期申请人在水稻中鉴定到一个新的耐低温QTL基因COLD6，它也编码膜蛋白，但其在低温感知中的分子机制仍不清楚，特别是与COLD1复合物的关系一无所知。本项目将在前期遗传位点研究的基础上，重点以COLD6为研究对象，通过遗传学等手段，明确COLD6与水稻耐寒的关系，分析其在低温信号调控中的功能；借助电生理实验，检测COLD6蛋白可能调控的第二信使；利用免疫共沉淀等手段筛选COLD6的互作蛋白，探索互作蛋白与COLD6共同调控低温信号的分子机理；借助生化、细胞及遗传学技术，分析COLD6与COLD1的功能相关性。通过本项目的研究将阐明COLD6调控水稻耐寒性的分子机制，建立和完善水稻低温信号感知和转导的调控网络。

Cold sensing is vital for initiating cold response and activating defense system in plants. However，the molecular mechanism of cold sensing is still less known. We have previously reported that COLD1, a plasma membrane protein, participated in cold signal perception and triggered downstream response to cold. We recently identify a new QTL for cold tolerance, COLD6, which also encodes a plasma membrane protein, but the molecular mechanism remain elusive, especially the relationship between COLD6 and COLD1 complex is still unknown. In this project, we will focus on COLD6 based on the previous studies on genetic loci, elucidate the relationship between COLD6 and cold tolerance and analyze the function of COLD6 in regulation of cold signaling by using genetics approaches, identify the COLD6-induced second messengers by voltage clamp and other experimental techniques, screen the interaction between COLD6 and other proteins, explore the molecular mechanism of interacting proteins and COLD6 to regulate cold signaling, as well as investigate the relationship between COLD6 and COLD1 with the help of biochemical, cellular and genetic experiments. Collectively, this project will elucidate the molecular mechanism of COLD6 to regulate chilling stress in rice, and establish the regulatory network of cold sensing and signal transduction.