MoWhi2调控MoUth1介导的线粒体自噬分子机制研究

稻瘟病菌引起的稻瘟病严重威胁粮食安全生产。线粒体自噬在稻瘟病菌分生孢子形成和侵染菌丝扩展中发挥着重要作用，但其受体及调控机制尚不清楚。申请人前期鉴定到一个控制稻瘟病菌产孢和致病的线粒体自噬基因MoWHI2，并获得了与MoWhi2互作的候选蛋白MoUth1，初步研究表明MoUth1可能通过与MoAtg8互作参与线粒体自噬。在此基础上，本项目将综合利用同源敲除、免疫共沉淀和线粒体自噬检测技术等手段：验证MoWhi2与MoUth1互作；分析MoUth1在致病过程中的功能及其是否作为受体参与线粒体自噬；阐明MoWhi2调控MoUth1介导的线粒体自噬进而控制产孢和致病的分子机制。预期研究结果将揭示稻瘟病菌线粒体自噬发生的分子机理，加深对稻瘟病菌致病机制的理解，为稻瘟病的防控提供重要参考。

Rice blast, caused by Magnaporthe oryzae, seriously threatens the safety of food production. Mitophagy plays important roles in conidiation and invasive growth of M. oryzae, but its receptor and regulatory mechanism are still unclear. In previous study, the applicant identified a mitophagy gene MoWHI2 controlling sporulation and pathogenicity of M. oryzae, and obtained a candidate protein MoUth1 interacting with MoWhi2. Our preliminary studies showed that MoUth1 may participate in mitophagy by interacting with MoAtg8. On this basis, this project will use one-step gene replacement strategy, Co-immunoprecipitation and mitophagy detection techniques to verify the interaction between MoWhi2 and MoUth1; explore the function of MoUth1 in the pathogenesis process and whether MoUth1 is involved in mitophagy as a receptor; reveal how MoWhi2 regulates MoUth1-medaited mitophagy to control the sporulation and pathogenic process. It is expected that the results will reveal the molecular mechanism of mitophagy, deepen the understanding of the pathogenic mechanism of M. oryzae, and provide an important reference for the prevention and control of rice blast.