雷公藤红素抑制强直性脊柱炎易感基因HLA-B27表达的作用机制研究

雷公藤是临床常用治疗强直性脊柱炎（AS）药物，具体作用机制尚不清楚。人类白细胞抗原HLA-B27是已知的与AS发病最为密切的基因。我们前期研究发现雷公藤红素对HLA-B27启动子活性有明显抑制作用，提示从HLA-B27着手可能是揭示雷公藤作用新靶点。本研究通过HLA-B27转基因鼠、HLA-B27荧光素酶报告载体、Real-time PCR、ELISA、Micro-CT等技术分别通过动物模型和细胞模型，观察雷公藤主要成分雷公藤红素对HLA-B27基因表达的调控作用。本研究将不仅可以为揭示雷公藤治疗AS的分子机制提供新的依据，而且此研究策略也可有助于进一步筛选其他调节HLA-B27表达进而控制AS发生、发展的中药或化合物。

Celastrol is commonly used in clinical treatment of ankylosing spondylitis (AS), the specific mechanism is still unclear.HLA-B27 is the most closely related gene with AS.Our previous study found that celastrol had significantly inhibition on the HLA-B27 promoter activity,suggesting that proceed from the HLA-B27 may reveal a new target of celastrol role.In this study,we will observe celastrol ,the main ingredients of Tripterygium ,the regulating role on HLA-B27 gene expression in animal models and cell models, with the methods of HLA-B27 transgenic mice、HLA-B27 luciferase reporter vector, Real-time PCR ELISA,the Micro-CT, etc. This study will not only provide a new basis to reveal the molecular mechanisms of the Tripterygium treatment of AS, but this research strategy may also help to further filter the other Herbs or compounds ,which can regulate the expression of HLA-B27 and control the AS occurred.