制备一种含有磁性纳米颗粒的聚甲基丙烯酸甲酯骨水泥，载有R837（一种强效的Toll样受体7兴奋剂）在交变磁场中对肿瘤产生“肿瘤疫苗”样作用。在此基础上辅以细胞毒性T细胞相关蛋白4（CTLA4）免疫检查点阻断法通过对T细胞的调节进一步加强抗肿瘤免疫反应。用“手术＋载有R837的磁性骨水泥＋CTLA4免疫检查点阻断法”对兔VX-2骨转移瘤模型在外加磁场下进行治疗。对于治疗14天后出现复发的兔子采用加注载有R837的磁性纳米颗粒，观测兔子的生存时间和存活率。这种“手术＋载有免疫佐剂的磁性骨水泥”＋“CTLA4免疫检查点阻断法”＋“载有免疫佐剂的磁流体”的三联疗法可能具有安全，经济，高效的特点，不仅能有效地引起体内抗肿瘤免疫反应而且能治疗由于骨转移瘤引起的病理性骨折，减轻了患者的痛苦，为临床治疗骨转移瘤提供了一种新的思路。

A new therapeutic strategy that can inhibit metastatic tumor of bone will be developed by combining adjuvant magnetic polymethylmethacrylate(PMMA) bone cement-based magnetic hyperthermia with checkpoint-blockade immunotherapy .Imiquimod (R837), a Toll-like-receptor-7 agonist, magnetic nanoparticle(MNP) and PMMA are assembled .The formed R837-PEG-MNPs-PMMA bone cenment placed in an alternating magnetic field can generate tumour-associated antigens ,showing tumor vaccine-like functions. In combination with the checkpoint-blockade immunotherapy using anti-cytotoxic T-lymphocyte antigen-4 (CTLA4), the generated immunological responses will be able to attack remaining tumour cells in rabbits, effective in metastasis inhibition.14 days after treatments ,rabbits which occur tumour recurrence are injected with R837-PEG-MNPs then survival time and viability are assessed .The triple therapy, using ’operation and R837-PEG-MNPs-PMMA placed in an alternating magnetic field’+ ‘CTLA4 checkpoint-blockade immunotherapy’+ ‘R837-PEG-MNPs magnetofluid’ ,can not only treat pathologic fracture but also inhibit metastasis,which may provide a new therapeutic strategy to cure metastatic tumour of bone.