牛传染性鼻气管炎病毒（IBRV）感染牛后引起高热、鼻气管炎、流产等主要临床症状。IBRV导致的免疫抑制、潜伏感染、持续感染是IBR防控的主要障碍，严重影响畜牧业健康和可持续性发展。病毒诱导的宿主细胞线粒体损伤在病毒性疾病的发生发展过程中起着至关重要的作用。有研究证实，IBRV感染引起牛肾细胞（MDBK）线粒体损伤的同时非编码RNA表达谱发生了变化，但是差异表达的非编码RNA在IBRV诱导细胞线粒体损伤过程中的具体作用及机制，迄今尚不清楚。本项目拟采用高通量测序技术检测IBRV诱导MDBK细胞线粒体损伤过程中细胞内circRNA、miRNA、mRNA表达谱的变化，采用生物信息学技术筛选、预测并鉴定线粒体相关circRNA，确定circRNA在IBRV诱导线粒体损伤过程中的作用及机制。预期研究结果为进一步阐明IBR致病机制提供理论依据，为研发抗IBR药物提供新的分子靶标。

Infectious bovine rhinotracheitis virus （IBRV）infection causes high fever, rhinotracheitis, abortion and other clinical symptoms in cattle. The virus's immunosuppressive effect, latent infection and persistent infection are the main obstacles to the prevention and control of IBR, which seriously affect the healthy and sustainable development of animal husbandry. However, the pathogenic mechanism of IBRV infection have not been well understood, and it needs further research and exploration. Host cell mitochondrial damage caused by viruses plays a pivotal role in the process of generation and development of viral diseases. Studies have confirmed that IBRV infection causes mitochondrial damage and changes in non-coding RNA expression profiles of bovine kidney cells (MDBK), so we presume that the differential-expressed non-coding RNAs may be involved in the process of mitochondrial damage. In this project, we will detect the circRNA, miRNA and mRNA expression profile changes of MDBK cells by using high-throughput sequencing technique during mitochondrial damage process induced by IBRV. Then we will use bioinformatics technology to screen, predict and identify the circRNAs related to mitochondrion based on integrative analysis of circRNA, miRNA and mRNA expression, and determine the function and mechanism of the circRNA in mitochondrial damage. The results are expected to provide a theoretical basis for further elucidating the pathogenesis of IBR and new molecular targets for research and development of new medicine against IBRV.