Membranes of animal cells. I. Methods of isolation of the surface membrane
Membranes of animal cells. I. Methods of isolation of the surface membrane
复制标题
动物细胞膜。
DOI：
10.1002/jcp.1040680308
复制
发表时间：
1966
影响因子：
5.6
通讯作者：
M. M. Nass
中科院分区：
文献类型：
--
作者：
L. Warren;M. Glick;M. M. Nass
Procedures have been devised for the isolation of the surface membranes of mouse fibroblasts (L cell) and a variety of other cells. The surface membranes are stabilized by various reagents in a hypotonic solution and are then removed intact or as large fragments with a Dounce homogenizer. The membranes are purified by differential centrifugation on solutions of sucrose or glycerol or on a column of fine glass beads. A trilaminar pattern can be seen in thin sections of the membrane in the electron microscope. Sufficient material can be conveniently obtained for chemical analyses.
