Effects of recirculating flow on U-937 cell adhesion to human umbilical vein endothelial cells
Effects of recirculating flow on U-937 cell adhesion to human umbilical vein endothelial cells
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DOI：
10.1152/ajpheart.1998.275.2.h591
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发表时间：
1998-08-01
影响因子：
4.8
通讯作者：
Truskey, GA
中科院分区：
文献类型：
--
作者：
Barber, KM;Pinero, A;Truskey, GA
我们使用突然扩张的流动室来检测U-937细胞与未激活的和肿瘤坏死因子(TNF)α激活的人脐静脉内皮细胞(HUVEC)在循环流中的粘附性。对于未激活和肿瘤坏死因子-α激活的HUVEC，U-937细胞在类似于150微米的血流再附着范围内表现出一过性的停滞。几乎没有人直接在重新附着的地方被捕。未观察到U-937细胞滚动。在回流区内的所有其他位置，U-937电池没有表现出短暂的停滞或滚动。肿瘤坏死因子-α的激活增加了U-937细胞在接近再附着时的停滞频率，但不改变中位停滞时间。数值模拟的细胞轨迹未能预测再附着点附近的附着点。实验与理论的偏差可能是由于U-937细胞的非球形和变形性造成的。这些结果表明，U-937细胞的瞬时停滞优先发生在回流中再附着点附近。粘连的可能机制包括低剪应力、弯曲的流线、垂直于内皮的流体速度分量以及形成更大的接触区。
We used a sudden-expansion flow chamber to examine U-937 cell adhesion to unactivated and tumor necrosis factor (TNF)-alpha-activated human umbilical vein endothelial cells (HUVEC) in recirculating flow. For both unactivated and TNF-alpha-activated HUVEC, U-937 cells exhibited transient arrests within similar to 150 mu m of flow reattachment. Few arrests occurred directly at the reattachment site. U-937 cell rolling was not observed. At all other locations within the recirculation zone, U-937 cells did not exhibit transient arrests or rolling. TNF-alpha activation increased the frequency of U-937 cell arrests near reattachment but did not change the median arrest duration. Numerically simulated cell trajectories failed to predict attachment near the reattachment point. Deviations between experiment and theory may result from the nonspherical shape and deformability of U-937 cells. These results demonstrate that U-937 cell transient arrests occur preferentially in the vicinity of the reattachment point in recirculating flow. Possible mechanisms for adhesion include low shear stress, curved streamlines, fluid velocity components normal to the endothelium, and formation of larger contact areas.