Pseudonocardia dioxanivorans sp nov., a novel actinomycete that grows on 1,4-dioxane
Pseudonocardia dioxanivorans sp nov., a novel actinomycete that grows on 1,4-dioxane
复制标题
DOI：
10.1099/ijs.0.63085-0
复制
发表时间：
2005-03-01
影响因子：
2.8
通讯作者：
Alvarez-Cohen, L
中科院分区：
文献类型：
--
作者：
Mahendra, S;Alvarez-Cohen, L
从1，4-二氧六环污染的工业污泥中分离到一株放线菌（CB 1190（T））。该培养物的细胞是革兰氏阳性的，并表现出分枝的气生和营养菌丝体。16 S rRNA基因序列分析表明，该菌株属于假诺卡氏菌属，与烃氧化假诺卡氏菌、氧化硫化假诺卡氏菌和嗜盐假诺卡氏菌密切相关。CB 1190 T的生理生化特性与已知的假诺卡氏菌属其他种不同。这里描述的新生物的特点是能够在1，4-二氧六环上生长，这是一种可能的人类致癌物。这种培养物也可以在四氢呋喃、汽油芳烃和其他几种有毒环境污染物上生长。菌株CB 1190 T具有固定双氮的能力。主要的脂肪酸是16：0异，16：1异顺9和17：1异顺9。主要的磷脂脂肪酸是16：0 iso、16：0 10-Me和17：0 10-Me。肽聚糖属于A1型γ，内消旋二氨基庚二酸。主要的甲基萘醌是MK-8（H-4）-不存在分枝菌酸。G + C含量为74摩尔%。根据形态学、生理学、化学分类学和系统发育学等方面的证据，认为菌株CB 1190（T）（=ATCC 55486（T）=DSM 44775（T））被分类为一个新物种的模式菌株，食二氧杂环假诺卡氏菌（Pseudonocardia dioxanivorans sp. nov.）。环境中的4-二氧六环。
An actinomycete strain (CB1190(T)) was previously isolated from industrial sludge contaminated with 1,4-dioxane. The cells of this culture are Gram-positive and exhibit branching aerial and vegetative mycelium. Analysis of the 16S rRNA gene sequence indicates that the strain belongs to the genus Pseudonocardia, closely related to Pseudonocardia hydrocarbon oxydans, P. sulfidoxydans and P. halophobica. Physiological and biochemical characteristics of CB1190T are different from those of other known Pseudonocardia species. The novel organism described here is distinguished by its ability to grow on 1,4-dioxane, which is a probable human carcinogen. This culture can also grow on tetrahydrofuran, gasoline aromatics and several other toxic environmental contaminants. Strain CB1190T is capable of fixing dinitrogen. The predominant fatty acids are 16 : 0 iso, 16: 1 iso cis9 and 17 : 1 iso cis9. The major phospholipid fatty acids are 16: 0 iso, 16: 0 10-Me and 17: 0 10-Me. The peptidoglycan belongs to type A1 gamma, meso-diaminopimelic acid. The major menaquinone is MK-8 (H-4)- Mycolic acids are absent. The G + C content is 74 mol%. Based on morphological, physiological, chemotaxonomic and phylogenetic evidence, it is proposed that strain CB1190(T) (=ATCC 55486(T) =DSM 44775(T)) be classified as the type strain of a novel species, Pseudonocardia dioxanivorans sp. nov. Further studies with this organism will provide insights into metabolic pathways, responsible enzymes, kinetics and the fate of 1,4-dioxane in the environment.