ENKEPHALIN-CONTAINING AMACRINE CELLS IN THE AVIAN RETINA - IMMUNOHISTOCHEMICAL LOCALIZATION
ENKEPHALIN-CONTAINING AMACRINE CELLS IN THE AVIAN RETINA - IMMUNOHISTOCHEMICAL LOCALIZATION
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DOI：
10.1073/pnas.76.6.3010
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发表时间：
1979-01-01
影响因子：
11.1
通讯作者：
LAVERACK, C
中科院分区：
文献类型：
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作者：
BRECHA, N;KARTEN, HJ;LAVERACK, C
采用[Met5]脑啡肽和[Leu5]脑啡肽抗血清免疫荧光和免疫过氧化物酶技术研究了脑啡肽样免疫反应性在鸟[鸽]视网膜内的分布。用合成[Met5]-或[Leu5]脑啡肽在抗血清中吸收抗血清，在1 mM处对甲醛固定视网膜进行切片和抗血清培养。在无突细胞体细胞及其内丛状层突起中观察到脑啡肽免疫组化染色阳性。大量含脑啡肽的无突细胞分布于整个视网膜，其密度在视网膜中央区域最大。标记的无毛细胞大部分在7.5 μ左右。直径约为M，偶见无毛细胞，直径约为12-15 μ M。直径为M。无毛细胞的细胞间间距约为40亩。视网膜中央区域的M。观察到无突细胞的标记突向内丛状层突出，并在内丛状层的第1、3-5层内形成细丛。这些观察结果表明，阿片肽存在于视网膜内看似选择的无突细胞群中。脑啡肽样免疫反应性在视网膜内的定位表明，阿片肽在视网膜加工中起着特殊而独特的功能作用。
The distribution of enkephalin-like immunoreactivity within the avian [pigeon] retina was studied by immunofluorescence and immunoperoxidase techniques with antiserum to [Met5]enkephalin and [Leu5]enkephalin. Formaldehyde-fixed retinae were sectioned and incubated in antiserum was established by absorption of the antiserum with synthetic [Met5]- or [Leu5]enkephalin at 1 mM. Positive immunohistochemical staining for enkephalin was observed in the somata of amacrine cells and their processes within the inner plexiform layer. A large number of enkephalin-containing amacrine cells were distributed throughout the retina, and their density appeared to be greatest within central retinal regions. The majority of labeled amacrine cells were about 7.5 .mu.m in diameter although, occasionally, amacrine cells were observed that were 12-15 .mu.m in diameter. Amacrine cells had a cell-to-cell spacing of approximately 40 .mu.m within central retinal regions. Labeled processes of the amacrine cells were observed to project into the inner plexiform layer where they arborized as a fine plexus, within laminae 1, 3-5 of the inner plexiform layer. These observations demonstrate the existence of opioid peptides in seemingly select populations of amacrine cells within the retina. The localization of enkephalin-like immunoreactivity within the retina suggests that opioid peptides play a specific and unique functional role in retinal processing.