GhHB12, a HD-ZIP I Transcription Factor, Negatively Regulates the Cotton Resistance to Verticillium dahliae
GhHB12, a HD-ZIP I Transcription Factor, Negatively Regulates the Cotton Resistance to Verticillium dahliae
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DOI：
10.3390/ijms19123997
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发表时间：
2018-12-01
影响因子：
5.6
通讯作者：
Zhu, Longfu
中科院分区：
文献类型：
--
作者：
He, Xin;Wang, Tianyi;Zhu, Longfu
同源结构域亮氨酸拉链（homeodomain-leucine zipper，HD-ZIP）是一类植物特异性转录因子家族，在植物发育过程中起重要作用。我们先前分离到棉花HD-ZIP I类转录因子基因GhHB 12，该基因受昼夜节律钟和光周期调节。此外，它还调节棉花的结构、相变和光周期敏感性。在这里，我们报告的GhHB 12诱导茉莉酸甲酯（MeJA）和大丽轮枝菌感染。此外，在GhHB 12启动子中发现了胁迫响应元件。启动子融合分析表明，GhHB 12主要在初生根中表达，它是由机械损伤诱导的。GhHB 12的过表达增加了棉花植物对真菌病原体灰葡萄孢和大丽轮枝孢的易感性，这与茉莉酸（JA）响应基因GhJAZ 2和GhPR 3的抑制相结合。我们的研究结果表明，GhHB 12，棉花胁迫响应HD-ZIP I转录因子，负调控棉花抗病性大丽轮枝菌抑制JA-反应基因。
The homeodomain-leucine zipper (HD-ZIP) is a plant-specific transcription factor family that plays important roles in plant developmental processes in response to multiple stressors. We previously isolated a cotton HD-ZIP class I transcription factor gene, GhHB12, which is regulated by the circadian clock and photoperiodism. Furthermore, it regulates cotton architecture, phase transition, and photoperiod sensitivity. Here we report that GhHB12 was induced by methyl jasmonate (MeJA) and Verticillium dahliae infection. Additionally, stress-responsive elements were found in the GhHB12 promoter. Promoter fusion analysis showed that GhHB12 was predominantly expressed in primary roots and that it was induced by mechanical damage. Overexpression of GhHB12 increased susceptibility of the cotton plant to the fungal pathogens Botrytis cinerea and V. dahliae, which was coupled with suppression of the jasmonic acid (JA)-response genes GhJAZ2 and GhPR3. Our results suggest that GhHB12, a cotton stress-responsive HD-ZIP I transcription factor, negatively regulates cotton resistance to V. dahliae by suppressing JA-response genes.